多肽-2与阿尔茨海默病β-分泌酶特异性结合的研究  

Study on peptide-2 specifically binding to β-secretase of Alzheimer's disease

作　　者：朱瑞 金丽娟 高小芳 王方[2] ZHU Rui;JIN Lijuan;GAO Xiaofang;WANG Fang(ICU,Ningxia People＇s Hospital,Ningxia 750002,China;Department of Neurobiology,Southern Medical University,Guangdog 510515,China)

机构地区：[1]宁夏人民医院ICU,宁夏银川750002 [2]南方医科大学神经生物学教研室,广东广州510515

出　　处：《宁夏医学杂志》2018年第9期792-794,I0001,共4页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(NZ16178)

摘　　要：目的在细胞水平上鉴定多肽-2(Peptide-2)与阿尔茨海默病β-分泌酶(BACE1)结合的特异性。方法合成经过二硫键修饰的Peptide-2,采用激光扫描共聚焦显微镜术及流式细胞术鉴定Peptide-2对表达BACE1的人源胚胎肾293(HEK293)细胞的结合特异性。结果激光扫描共聚焦显微镜显示Peptide-2在表达BACE1的HEK293细胞上有高富集荧光亮点(红色荧光),流式细胞术鉴定出Peptide-2与HEK293细胞中BACE1结合的荧光强度值为102. 352,PBS对照组的荧光强度值为16. 29,差异有统计学意义(P <0. 05)。结论 Peptide-2在细胞水平能特异结合至BACE1,为阿尔茨海默病β-分泌酶抑制剂的筛选提供了一个新的研究方向。Objective To identify the specific peptide-2 binding on the surface of β-secretase（BACE1） at cellular level,which was screened by phage display peptide library through preliminary experiment. Methods The peptide-2 was synthetized by chemical methods after modification by sulfur bonds. The specificity of peptide-2 binding to BACE1 on HEK293 cell was identified by confocal laser microscope and flow cytometry. Results The confocal laser scanning microscope identified that the peptide-2 could bind to BACE1 on HEK293 cell with high fluorescence（red）. The fluorescence value of peptide-2 bind to BACE1 was 102. 352,and the fluorescence value of PBS control was 16. 29（P 0. 05） by flow cytometry. Conclusion The small molecular peptide named peptide-2 binding to BACE1 on HEK293 cell specifically could provide a new direction for screening β-secretase inhibitor candidate of Alzheimer＇s disease.

关 键 词：阿尔茨海默病 Β-分泌酶 特异性结合肽 

分 类 号：R737[医药卫生—肿瘤]