基于PG蛋白的蔷薇科植物分子进化树的构建与分析  

Construction and Analysis of Molecular Phylogenetic Tree of Rosaceae Plant Based on Polygalacturonase Protein

作　　者：李芳菲 马文瑶[1] 金亮亮 杨英军[1] Li Fangfei;Ma Wenyao;Jin Liangliang;Yang Yingjun(College of Forestry,Henan University of Science and Technology,Luoyang,471023)

机构地区：[1]河南科技大学林学院,洛阳471023

出　　处：《分子植物育种》2018年第19期6332-6340,共9页Molecular Plant Breeding

基　　金：河南省教育厅重点项目(13A210873);洛阳市科技发展计划项目(1402011A)共同资助

摘　　要：为了分析蔷薇科植物PG蛋白序列的进化关系,从NCBI网站搜索到177条相关蔷薇科植物PG蛋白序列,筛选出23条可用序列,采用ClustalW2和MEGA6两种聚类方法联配,并构建分子进化树。结果表明:蔷薇科植物23条PG序列聚类成2个类群,一类群有14条序列,主要为苹果亚科梨属和苹果属植物;另一类群包括8条序列,为李亚科的桃属和杏属植物。ClustalW2和MEGA6两种方法的分析结果基本一致,表明两种方法都可用于评价蔷薇科植物的演化与进化关系。本研究通过对23条蔷薇科植物PG序列的亲缘关系的分析,为蔷薇科植物在基因工程育种方面提供了理论依据。In order to analyze the evolutionary relationship of PG protein sequences from Rosaceae family, this research obtained 177 PG from the NCBI web site, screened 23 complete available sequences, and constructed molecular dendrograms by using ClustalW2 and MEGA6 methods. The results showed that 23 PG protein se- quences could be mainly divided into two groups, the first group included 14 sequences, which belongs to Maloideae and Malus; The other groups including 8 sequences, which belongs to the Armeniaca Mill A mygdalus L. of Prun- oideae. The results of both ClustalW2 and MEGA6 were basically the same, and they could be used to analyze hylo- genetic relationship based on PG sequences. In this study, the genetic relationship of PG sequences of 23 Rosaceae plants was analyzed, which might provide theoretical basis for genetic engineering breeding of Rosaceae plants.

关 键 词：蔷薇科 多聚半乳糖醛酸酶 分子进化树 亲缘关系 

分 类 号：Q941[生物学—植物学]