Mi R-31在胃癌组织中低表达并抑制HGC-27细胞增殖、迁移与侵袭  

MiR-31 is poorly expressed in gastric cancer tissues and inhibits proliferation, migration and invasion of HGC-27 cells

作　　者：苏宇 宋刚 张晓农 刘丽波[3] SU Yu;SONG Gang;ZHANG Xiao-nong;LIU Li-bo(Second Department of General Surgery;Department of Interventional Vascular Surgery,Panjin Central Hospital,Panjin 124010,China;Department of Neurobiology,China Medical University,Shenyang 110001,China)

机构地区：[1]盘锦市中心医院普外二科,辽宁盘锦124010 [2]盘锦市中心医院介入血管外科,辽宁盘锦124010 [3]中国医科大学神经生物学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2018年第5期469-471,475,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(30670723)

摘　　要：目的研究miR-31在胃癌患者中的表达,探讨miR-31对胃癌HGC-27细胞增殖、侵袭和转移的影响。方法收集胃癌患者标本39例,同时收取相应的癌旁组织20例。通过荧光定量PCR方法检测各组织中miR-31的表达水平。以脂质体为载体将miR-31模拟物转染至HGC-27细胞,CCK-8方法检测细胞增殖能力变化,Transwell实验检测HGC-27细胞侵袭和迁移能力变化。结果 miR-31在胃癌组织中的表达水平明显低于癌旁组织,P<0.01。过表达miR-31能够显著抑制HGC-27细胞的增殖,显著抑制HGC-27细胞的侵袭和迁移能力(P<0.01)。结论 MiR-31在胃癌中低表达,并抑制胃癌细胞系HGC-27的增殖、迁移和侵袭。Objective To investigate the expression of miR-31 in gastric cancer patients and explore the effect of miR-31 on the proliferation, invasion and metastasis of gastric cancer HGC-27 cells. Methods A total of 39 patients with gastric cancer was collected, while 20 corresponding para-cancerous tissues were collected. The expression level of miR-31 in tissues was detected by fluorescence quantitative PCR（qRT-PCR）. The miR-31 mimics were transfected into HGC-27 cells with liposome, and cell proliferation was detected by CCK-8 method. The invasion and migration abiity of HGC-27 cells were detected by transwell test. Results The expression level of miR-31 in gastric cancer tissues was significantly lower than that in adjacent tissues（P〈0.01）. The over-expression of miR-31 could significantly inhibit the proliferation, invasion and migration of HGC-27 cells. Conclusion MiR-31 is poorly expressed in gastric cancer and inhibits proliferation, migration and invasion of gastric cancer cell line HGC-27.

关 键 词：miR-31 胃癌 增殖 侵袭 迁移 HGC-27细胞 

分 类 号：R735.2[医药卫生—肿瘤]