扁塑藤素通过调控自噬促进人胶质母细胞瘤U251细胞凋亡  

Pristimerin Romotes Cell Poptosis of Uman Lioblastoma U251 Ells by Nhibiting Utophagy

作　　者：徐高峰[1] 鲍钢 白晓斌[1] 祁磊[1] 谢万福[1] 李瑞春[1] XU Gao-feng;BAO Gang;BAI Xiao-bin;QI Lei;XIE Wan-fu;LI Rui-chun(Department of Neurosurgery,The First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China)

机构地区：[1]西安交通大学医学院第一附属医院神经外科,陕西西安710061

出　　处：《标记免疫分析与临床》2018年第10期1530-1535,共6页Labeled Immunoassays and Clinical Medicine

基　　金：陕西省科技研究发展(攻关)计划项目(编号:2014K11-01-01-03)

摘　　要：目的探讨扁塑藤素对人胶质母细胞瘤U251细胞凋亡的影响。方法 2μmol/L、4μmol/L和8μmol/L的扁塑藤素处理U251细胞24 h后,用四甲基偶氮唑蓝(MTT)法检测U251细胞活力;Annexin V-PI双染法检测U251细胞凋亡率;免疫印迹法检测促凋亡蛋白Bax和自噬相关蛋白Beclin1和LC3的表达水平。结果 2μmol/L、4μmol/L和8μmol/L的扁塑藤素呈浓度依赖性地降低U251细胞活力。扁塑藤素处理U251细胞可显著增加凋亡细胞数量以及上调Bax蛋白表达水平。本研究还发现扁塑藤素处理后,U251细胞Beclin1蛋白表达水平以及LC3II/LC3I的比值明显降低。结论扁塑藤素可能通过抑制自噬促进人胶质母细胞瘤的细胞凋亡。Objective To explore the influence of pristimerin on cell apoptosis of U251 cells. Methods After treated with different concentrations of pristimerin （2 μ mol/L,4 μ mol/L,8 μ mol/L）for 24 h,the viability of U251 cells was detected using MTT assay; the rate of cell apoptosis was detected by flow cytometry （FCM）after AnnexinV- PI staining; the expression levels of pro- apoptosis associated protein Bax and autophagy related proteins Beclin1 and LC3 were determined by Western blot. Results Compared with the control group,pristimerin significantly decreased the viability of U251 cells in a dose dependent manner.Furthermore,the cell apoptosis rate and the expression level of Bax protein in pristimerin- treated U251 cells were significantly elevated.We also observed that the levels of autophagy related proteins Beclin1 and LC3II/LC3I were decreased in U251 cells treated by pristimerin. Conclusion Pristimerin can promote cell apoptosis of human glioblastoma,which might be associated with the inhibition of autophagy.

关 键 词：扁塑藤素 胶质母细胞瘤 细胞凋亡 自噬 

分 类 号：R739.41[医药卫生—肿瘤]