染料木素对HT29细胞IL-8分泌及Akt/NF-κB活化的影响  

Effect of Genistein on Akt/NF-κB pathway mediated IL-8 secretion in HT29 cells

作　　者：徐成飞 韦立群 李通[1] 潘晓航 甘嘉亮[1] Cheng-fei Xu;Li-qun Wei;Tong Li;Xiao-hang Pan;Jia-liang Gan(The First Affiliated Hospital of Guangxi Medical University,Nanning,Guangxi 530021,China)

机构地区：[1]广西医科大学第一附属医院,广西南宁530021

出　　处：《中国现代医学杂志》2018年第30期11-15,共5页China Journal of Modern Medicine

基　　金：广西自然科学基金面上项目(No:2013GXNSFAA019153);广西高校科学技术研究项目(No:2013ZD015)

摘　　要：目的观察染料木素对脂多糖(LPS)和人干扰素γ(hIFN-γ)诱导人结肠癌HT29细胞诱导产生白细胞介素8(IL-8)中Akt/NF-κB信号通路的作用,并探讨其可能的作用机制。方法用噻唑蓝(MTT)法检测染料木素对HT29细胞增殖的影响。用实时荧光定量聚合酶链反应(qRT-PCR)检测白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、IL-8、白细胞介素10(IL-10)mRNA表达水平,酶联免疫吸附法(ELISA)检测上清中IL-8的含量。Western blot检测细胞p-Akt(S473)、Akt、p-p65、p65蛋白的表达情况。结果用量达200μmol/L的染料木素处理HT29细胞,未对细胞的增殖产生影响。ELISA与qRT-PCR证实染料木素能降低LPS+hIFN-γ诱导HT29细胞转录和分泌IL-8。Western blot结果表明,染料木素可以抑制LPS+hIFN-γ诱导的Akt/NF-κB的活化。结论染料木素能够抑制LPS+hIFN-γ诱导的HT29中IL-8的转录和分泌发挥抗炎作用,可能与抑制Akt/NF-κB通路的活化有关系。Objective To investigate the effect of Genistein on Akt/NF-κB pathway mediated interleukin-8（IL-8） secretion in human colon carcinoma HT29. Methods Cells were treated with LPS plus hIFN- γ.Cellular proliferation of HT29 cells was measured by thiazolyl blue tetrazolium bromide （MTT） test. Expression of interleukin-1β （IL-1β）, interleukin-6 （IL-6）, IL-8 and interleukin-10 （IL-10） was identified by quantitative real timepolymerase chain reaction （qRT-PCR） and enzyme-linked immunosorbent assay （ELISA）. Western blot method was performed to detect the expression of p-Akt （S473）, Akt, p-p65 and p65. Results Genistein （200 μmol/L） had no obvious inhibitory effect on HT29 cell proliferation. ELISA and qRT-PCR confirmed reduction of LPS＋hIFN-γinduced IL-8 in Genistein treated HT29. Western blot analysis indicated that Genistein inhibited the activation of Akt/NF-κB induced by LPS plus hIFN-γ. Conclusion Genistein inhibits expression of IL-8 through inhibiting the activation of Akt/NF-κB pathway in HT29 cell line.

关 键 词：白细胞介素8 染料木素 炎性肠病 克罗恩病 溃疡性结肠炎 

分 类 号：R574[医药卫生—消化系统]