miR-379—5p对膀胱癌细胞增殖、迁移和侵袭的影响  

Effects of miR-379-Sp on proliferation, migration and invasion of bladder cancer cells

作　　者：陈昆[1] 邹建华[2] Chen Kun1, Zou Jianhua2.(1 Department of Urology, People＇s Hospital of Shifang City, Deyang 618400, China ;2 Department of Urology, People＇s Hospital of Sichuan Province, Deyang 618400, China)

机构地区：[1]德阳什邡市人民医院泌尿外科,四川618400 [2]德阳四川省人民医院泌尿外科,四川618400

出　　处：《国际泌尿系统杂志》2018年第5期741-744,共4页International Journal of Urology and Nephrology

摘　　要：目的 探讨微小核糖核酸-379-5p（miR-379-5p）对膀胱癌细胞增殖、迁移和侵袭的影响.方法 通过实时荧光定量PCR （qRT-PCR）法检测膀胱癌组织细胞与正常组织细胞中miR-379-5p表达水平,分别将miR-379-5p mimics序列和miR-NC序列转染至人膀胱移行细胞癌细胞系um-uc-3,作为miR-379-5p mimics组和miR-NC组,通过Western blot实验检测转染效率,采用MMT实验和Transwell实验比较两组um-uc-3细胞增殖、迁移以及侵袭能力.结果 qRT-PCR检测结果显示,膀胱癌组织中miR-379-5p mRNA相对表达量为（0.52 ±0.38）,显著低于正常组织中miR-379-5p mRNA相对表达量（1.00±0.51,P＜0.05）,um-uc-3中miR-379-5p mRNA相对表达量为（0.45±0.23）,显著低于人膀胱上皮永生化细胞SV-HUC-1中miR-379-5p mRNA相对表达量（1.00±0.14,P＜0.05）;Western blot结果显示,miR-379-5p mimics组miR-379-5p表达量升高,转染有效;MTI实验显示,miR-379-5pmimics组细胞在不同时间点OD值均小于miRNC组;Transwell实验显示,miR-379-5p mimics组细胞迁移和侵袭数量均显著少于miR-NC组（P＜0.05）.结论 miR-379-5p在膀胱癌组织细胞中表达均下调,体外上调miR-379-5p表达可有效抑制膀胱癌细胞的增殖、迁移和侵袭能力.Objective To explore the effects of micro ribonucleic acid-379-5p （miR-379-5p） on the proliferation,migration and invasion of bladder cancer cells.Methods The expression level of miR-379-5p in bladder cancer tissues and normal tissues was detected by real-time quantitative PCR （qRT-PCR）,and the miR-379-5p mimics sequence and miR-NC sequence were transfected into human bladder transitional cell carcinoma um-uc-3 cell and taken as the miR-379-5p mimics group and the miRNC group respectively.The transfection efficiency was detected by Westem blot,and the proliferation,migration and invasion of um-uc-3 cells were compared by MMT and Transwell experiments.Results The relative expression of miR-379-5p mRNA in the bladder cancer tissues was significantly lower than that in the normal tissues [（0.52 ± 0.38） vs.（1.00 ± 0.51）,P 〈 0.05],and the relative expression of miR-379-5p mRNA in the um-uc-3 was significantly lower than that in human bladder epithelium immortalized cells SV-HUC-1 [（0.45 ± 0.23） vs.（1.00 ± 0.14）,P 〈 0.05].The results of Western blot showed that the expression of miR-379-5p in miR-379-5p mimics group was increased and the transfection was effective.The MTT assay showed that the cells OD value at different time points in the miR-379-5p mimics group was lower than that in the miR-NC group.The Transwell experiments showed that the number of cell migration and invasion in the miR-379-5p mimics group was significantly less than that in the miR-NC group （P 〈 0.05）.Conclusions The expression of miR-379-5p is down regulated in the bladder cancer tissues,and the external up-regulation of miR-379-5p expression can effectively inhibit the proliferation,migration and invasion of bladder cancer cells.

关 键 词：膀胱肿瘤 细胞增殖 细胞运动 肿瘤转移 

分 类 号：R737.14[医药卫生—肿瘤]