Suppression of m6A reader Ythdf2 promotes hematopoietic stem cell expansion  

作　　者：Zhenrui Li Pengxu Qian Wanqing Shao Hailing Shi Xi C. He Madelaine Gogol Zulin Yu Yongfu Wang Meijie Qi Yunfei Zhu John M. Perry Kai Zhang Fang Tao Kun Zhou Deqing Hu Yingli Han Chongbei Zhao Richard Alexander Hanzhang Xu Shiyuan Chen Allison Peak Kathyrn Hall Michael Peterson Anoja Perera Jeffrey S. Haug Tari Parmely Hua Li Bin Shen Julia Zeitlinger Chuan He Linheng Li 

机构地区：[1]Stowers Institute for Medical Research, Kansas City, MO 64110, USA [2]Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, USA [3]Center of Stem Cell and Regenerative Medicine, and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China [4]Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Institute of Hematology, Zhejiang University, Hangzhou 310058, China [5]Department of Chemistry, Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL 60637, USA [6]Howard Hughes Medical Institute, The University of Chicago, Chicago, IL 60637, USA [7]State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 211166, China [8]Department of Hematology, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, China [9]Tianjin Medical University School of Basic Medicine, Tian Jin 300070, China [10]Shanghai JiaoTong University School of Medicine, Shanghai 200025, China

出　　处：《Cell Research》2018年第9期904-917,共14页细胞研究（英文版）

摘　　要：Transplantation of hematopoietic stem cells （HSCs） from human umbilical cord blood （hUCB） holds great promise for treating a broad spectrum of hematological disorders including cancer. However, the limited number of HSCs in a single hUCB unit restricts its widespread use. Although extensive efforts have led to multiple methods for ex vivo expansion of human HSCs by targeting single molecules or pathways, it remains unknown whether it is possible to simultaneously manipulate the large number of targets essential for stem cell self-renewal. Recent studies indicate that/^-methyladenosine （m6A） modulates the expression of a group of mRNAs critical for stem cell-fate determination by influencing their stability. Among several m6A readers, YTHDF2 is recognized as promoting targeted mRNA decay. However, the physiological functions of YTHDF2 in adult stem cells are unknown. Here we show that following the conditional knockout （KO） of mouse Ythdf2 the numbers of functional HSC were increased without skewing lineage differentiation or leading to hematopoietic malignancies. Furthermore, knockdown （KD） of human YTHDF2 led to more than a 10-fold increase in the ex vivo expansion of hUCB HSCs, a flvefold increase in colony-forming units （CFUs）, and more than an eightfold increase in functional hUCB HSCs in the secondary serial of a limiting dilution transplantation assay. Mapping of m6A in RNAs from mouse hematopoietic stem and progenitor cells （HSPCs） as well as from hUCB HSCs revealed its enrichment in mRNAs encoding transcription factors critical for stem cell self-renewal. These re^A-marked mRNAs were recognized by Ythdf2 and underwent decay. In Ythdf2 KO HSPCs and YTHDF2 KD hUCB HSCs, these mRNAs were stabilized, facilitating HSC expansion. Knocking down one of YTHDF21s key targets, Tall mRNA, partially rescued the phenotype. Our study provides the first demonstration of the function of YTHDF2 in adult stem cell maintenance and identifies its important role in regulating HSC ex vivo expansion b

关 键 词：成年干细胞 造血 读者 VIVO mRNA HSC 稳定性 单个分子 

分 类 号：Q25[生物学—细胞生物学] Q462