苹果褪绿叶斑病毒辽宁分离物生物学和基因组研究  

Biology and complete genome of Apple chlorotic leaf spot virus Liaoning isolate

作　　者：张双纳 李正男[1] 张尊平[1] 范旭东[1] 任芳[1] 胡国君[1] 董雅凤[1] ZHANG Shuang-na, LI Zheng-nan , ZHANG Zun-ping, FAN Xu-dong, REN Fang, HU Guo-jun, DONG Ya-feng(National Center for Eliminating Viruses from Deciduous Fruit Tree, Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China)

机构地区：[1]中国农业科学院果树研究所国家落叶果树脱毒中心,辽宁兴城125100

出　　处：《植物病理学报》2018年第5期716-720,共5页Acta Phytopathologica Sinica

基　　金：中央级公益性科研院所基本科研业务费专项(1610182016017);中国农业科学院科技创新工程

摘　　要：苹果褪绿叶斑病毒（Apple chlorotic leaf spotvirus，ACLSV）属于乙型线形病毒科（Betaflexiviridae）纤毛病毒属（Trichovirus），基因组为正义单链RNA。含有约7500个核苷酸。编码3个部分重叠的开放阅读框。目前从GenBank数据库可以检索到19个ACLSV分离物的基因组序列。In the present work, we studied the genomic and biological properties of Apple chlorotic leaf spot virus （ACLSV） isolate XC-HF. The leaves of Malus domestica cv. Hanfu collected from Xingcheng, Lianoning province were used as plant material. The samples were subjected to RT-PCR and RACE techniques to amplify the complete genome of ACLSV XC-HF, followed by sequencing. The genome of ACLSV XC-HF consists of 7 557 nucleotides, encoding three overlapping open reading frames （ORFs） and shared nucleotide identities of 68.9%-84.4% with the other 19 ACLSV isolates published in GenBank. Phylogenetic analysis based on the complete genorne sequences indicated that ACLSV XC-HF was located in the group JB. Recombination analysis suggested a recombinant event at 1-338 nts of ACLSV XC-HF. The ACLSV XC-HF was inoculated to Chenopodium amaranticolor by sap inoculation and to Malus sylvestris cv. R12740-7A by double budding, respectively, and induced symptoms of chlorosis and slight leaf roll on infected plants.

关 键 词：苹果褪绿叶斑病毒 基因组序列 分离物 GENBANK数据库 生物学 辽宁 开放阅读框 ACLSV 

分 类 号：S432.41[农业科学—植物病理学]