肠道菌群变化对肠上皮细胞Myd88蛋白及炎症因子的影响  

Impact of intestinal flora variation on Myd88 protein and inflammatory factors in intestinal epithelial cells of rats

作　　者：王海昆[1] 崔旻[1] 姚萍[1] WANG Haikun;CUI Min;YAO Ping(Department of Gastroenterology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830054,China)

机构地区：[1]新疆医科大学第一附属医院消化科,新疆乌鲁木齐830054

出　　处：《中国微生态学杂志》2018年第9期1004-1007,1012,共5页Chinese Journal of Microecology

基　　金：国家自然科学基金(81760100)

摘　　要：目的研究肠道菌群变化对体外培养的正常大鼠肠黏膜上皮细胞Myd88蛋白水平及其下游TNF-α等炎症因子的影响。方法选取10只健康大鼠,实验室适应性喂养5d后继续常规喂养7d并处死,取结肠组织进行细胞培养得到原代肠黏膜上皮细胞,将所得细胞分为5组,分别用正常细胞培养液(A组)、正常大鼠肠腔菌溶液(B组)、正常大鼠黏膜菌溶液(C组)、溃疡性结肠炎大鼠肠腔菌溶液(D组)、溃疡性结肠炎大鼠黏膜菌溶液(E组)培养,通过蛋白质印迹法检测各组细胞中Myd88蛋白含量,通过酶联免疫吸附法检测TNF-α和IL-6含量。结果 D组、E组与A组比较,细胞中Myd88蛋白及TNF-α、IL-6水平显著增高(Ps<0.01),D、E组与B、C组比较Myd88蛋白及TNF-α、IL-6水平亦显著增高(Ps<0.01),而B组、C组相较于A组差异无统计学意义(Ps>0.05)。进一步比较D组与E组、B组与C组间Myd88蛋白及TNF-α、IL-6水平差异亦无统计学意义(Ps>0.0.5)。结论 (1)肠道菌群结构的改变可引起肠黏膜细胞Myd88通路的激活及炎症因子的释放。(2)肠道中细菌种类、数量、分布位置等因素的综合变化与肠黏膜炎症反应的发生关系密切。Objective To observe the impact of intestinal flora variation on the levels of Myd88 protein and inflammatory factors such as TNF-αin normal rat primary colonic epithelial cells cultured in vitro.Methods 10 healthy rats were sacrificed after 5 days of adaptive feeding followed by 7 days of normal feeding in laboratory;the colonic tissue were collected and the primary colonic epithelial cells were cultured in vitro.The cells were randomly divided into 5 groups and cultured with normal cell culture medium（group A）,normal rat intestinal cavity bacteria（group B）,normal rat intestinal mucosal bacteria（group C）,UC rat intestinal cavity bacteria（group D）and UC rat intestinal mucosal bacteria（group E）,respectively.Myd88 protein was detected with Western Blot,and TNF-αand IL-6 were detected by using ELLSA.Results The levels of Myd88 protein,TNF-αand IL-6 in group D and E significantly increased than in group A,B and C（Ps0.01）.But,compare with group A,the levels of Myd88 protein,TNF-αand IL-6 in group B and C were not significantly different（Ps0.0.5）.Further more,there were no significant differences（Ps0.0.5）in the levels of Myd88 and inflammatory factors between group B and C,and between group D and E.Conclusion（1）The variation of intestinal flora structure may activate Myd88 pathway and cause the release of inflammatory factors in intestinal epithelial cells.（2）The changes of bacterial species,quantity and location in intestinal tract have a close relationship with the occurrence of inflammatory reaction of intestinal mucosa.

关 键 词：肠道菌群 体外培养肠上皮细胞 MYD88 炎症 

分 类 号：R574.62[医药卫生—消化系统]