基于正交试验配合多药效指标综合评价半夏白术天麻汤治疗痰湿壅盛型高血压的有效组分配伍  

Comprehensive Evaluation of Compatibility of Effective Components in Banxia Baizhu Tianma Tang for Phlegm Dampness Hypertension Based on Orthogonal Test Combined with Multiple Pharmacodynamic Indexes

作　　者：徐男[1,2] 时海燕 王淑玲[4] 王亮[1] 张成博[2] 孙蓉 XU Nan;SHI Hai-yan;WANG Shu-ling;WANG Liang;ZHANG Cheng-bo;SUN Rong(Shandong Academy of Chinese Medicine,Ji＇nan 250014,China;Postdoctoral Mobile Station of Chinese Medicine,Shandong University of Traditional Chinese Medicine,Ji＇nan 250355,China;Shandong Provincial Qianfoshan Hospital,Ji＇nan 250014,China;College of Medicine,Hangzhou Normal University,Hangzhou 310012,China;Institute of Advanced Medical Sciences,Shandong University,Ji＇nan 250012,China)

机构地区：[1]山东省中医药研究院,济南250014 [2]山东中医药大学中医学博士后流动站,济南250355 [3]山东省千佛山医院,济南250014 [4]杭州师范大学医学院,杭州310012 [5]山东大学高等医学研究院,济南250012

出　　处：《中国实验方剂学杂志》2018年第21期7-13,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省中医药科技发展计划项目(2017-136);浙江省自然科学基金项目(LY16H270018);山东省重点研发计划项目(2017CXGC1308);山东省泰山学者工程专项(ts201511107)

摘　　要：目的：筛选半夏白术天麻汤治疗痰湿壅盛型高血压的有效组分及其最佳配比。方法：根据原方的基本配比,采用四因素四水平的正交试验配比方案,以血清总胆固醇（TC）,甘油三酯（TG）,高密度脂蛋白-胆固醇（HDL-C）,低密度脂蛋白-胆固醇（LDL-C）,血糖（Glu）,空腹血胰岛素（FINS）,一氧化氮（NO）,血清血管紧张素Ⅱ（Ang-Ⅱ）和内皮素（ET）的水平为评价指标,利用多指标综合评价对不同配比下疗效结果进行分析研究。结果：半夏白术天麻汤治疗痰湿壅盛型高血压的最佳有效组分配伍组合为多糖组分12.95 mg·kg（-1）,皂苷组分9.00 mg·kg（-1）,酚类组分14.50 mg·kg（-1）。结论：正交试验配合多药效指标综合评价确定有效组分配比的方法稳定可行,优选的活性组分配伍的药效优于半夏白术天麻汤原方,且服药量明显减小。Objective： To screen effective components of Banxia Baizhu Tianma Tang in treatment hypertension of phlegm dampness type and their best ratio. Method： According to the basic ratio of the original formula,the orthogonal test with four factors and four levels was used to measure and research efficacy results with different proportions and the comprehensive evaluation of multiple indexes,including the levels of total cholesterol（ TC）,triglyceride（ TG）,high-density lipoprotein cholesterol（ HDL-C）,low-density lipoprotein cholesterol（ LDL-C）,glucose（ Glu）,fasting blood insulin（ FINS）,nitric oxide（ NO）,serum angiotensin Ⅱ（ AngⅡ）and endothelin（ ET）. Result： The optimal combination of effective components in Banxia Baizhu Tianma Tang for phlegm dampness hypertension was 12. 95 mg·kg-1 of total polysaccharides,9. 00 mg·kg-1 of total saponins and14. 50 mg·kg-1 of total polyphenols. Conclusion： Orthogonal test combined with multiple pharmacodynamic indexes to determine the compatibility of these effective components is feasible and stable,efficacy of this optimized compatibility of active components is better than that of the original prescription of Banxia Baizhu Tianma Tang,and dosis is significantly decreased.

关 键 词：半夏白术天麻汤 正交试验 痰湿壅盛型高血压 组分配伍 总胆固醇 脂蛋白 血管紧张素 

分 类 号：R22[医药卫生—中医基础理论] R289[医药卫生—中医学]