十八烷基壳聚糖膜制备与体外凝血性能的研究  

Preparation of N-octadecyl chitosan films and its coagulation properties in vitro

作　　者：楚雪欣 杨健 黄姝杰 关静 荆妙蕾[1] CHU Xue-xin;YANG Jian;HUANG Shu-jie;GUAN Jing;JING Miao-lei(Department of Textile,Tianjin University of Technology,Tianjin 300387,China;Institute of Medical Support Technology,Institute of Systems Engineering,Academy of Military Sciences,Tianjin 300161,China;Research Center for Military Standard,Institute of Systems Engineering,Academy of Military Sciences,Beijing 100141,China)

机构地区：[1]天津工业大学纺织学院,天津300387 [2]军事科学院系统工程研究院卫勤保障技术研究所,天津300161 [3]军事科学院系统工程研究院军用标准研究中心,北京100141

出　　处：《军事医学》2018年第5期368-373,共6页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31570956)

摘　　要：目的制备N-十八烷基壳聚糖膜,研究其体外凝血性能。方法以壳聚糖(chitosan,CS)和十八醛为原料,还原氨法制备不同取代度(40%、20%、10%)的N-十八烷基CS,流延法分别制备CS膜和N-烷基CS膜,红外、元素分析和溶胀率检测表征材料的结构、组成和吸水性,MTT法评价材料的细胞毒性,体外凝血时间研究材料的凝血性能。结果制备出不同取代度的N-十八烷基CS,并成功制备成膜。CS膜和N-十八烷基CS膜均无细胞毒性。不同取代度的N-十八烷基CS膜的体外凝血性能均优于纯CS膜,其凝血性能随十八烷基取代度的增加而增强。结论与纯CS膜相比,N-十八烷基CS膜具有促凝作用,且取代度越大,材料的凝血效果越好,表现出良好的生物相容性,有望作为医用敷料使用。Objective To prepare N-octadecyl chitosan films and study its coagulation performance in vitro. Methods Using chitosan（CS） and octadeealdehyde as raw materials, N-octadecyl CS with different degrees of substitution （DS 40% , 20% and 10% ） was prepared by reductive reaction. Its structure, composition and water absorbency properties were characterized by infrared, elemental analysis and swelling ratio detection. CS and N-octadeeyl CS solution was cast to form films. The cytotoxieity and coagulation properties of N-oetadecyl CS were evaluated by MTI＂ method and coagulation time in vitro respectively. Results N-octadecyl CS with different degrees of substitution and the corresponding film were successfully prepared. Both the CS membrane and the N-octadecyl CS film had no toxicity. The in vitro coagulation effect of any N-octadecyl CS film was better than that of CS film. Furthermore, the blood clotting effect was enhanced with the increase in DS. Conclusion Compared with CS films, N-octadecyl CS films have obvious procoagulant effect. The greater the DS is, the better the coagulation effect. Besides good biocompatibility, N-octadecyl chitosan can potentially be used as a safe wound dressing material.

关 键 词：壳聚糖膜 烷基壳聚糖膜 体外凝血 细胞毒性 动态凝血时间 生物敷料 

分 类 号：R318.08[医药卫生—生物医学工程]