E2F8在胶质瘤组织的表达及其对胶质瘤细胞增殖的作用  

Expression of E2F8 in glioma tissues and its effect on proliferation of glioma cells

作　　者：邢振义[1] 张红赟[1] 孙来广[1] Xing Zhenyi;Zhang Hongyun;Sun Laiguang(Department of Neurosurgery,Xinxiang Central Hospital,Xinxiang 453002,China)

机构地区：[1]河南省新乡市中心医院神经外科,453002

出　　处：《中华实验外科杂志》2018年第10期1949-1951,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察E2F8在胶质瘤中的表达及其对胶质瘤细胞增殖作用。方法采用生物信息学分析TCGA不同级别胶质瘤患者E2F8的表达水平差异，收集我院收治的不同级别胶质瘤患者105例和12例正常脑组织，分别采用Westernblot、定量聚合酶链反应（qPCR）和免疫组织化学检测E2F8的表达水平差异，分析其表达与患者的预后意义。通过小干扰RNA（siRNA）沉默E2F8，采用细胞计数试剂盒（CCK-8）和集落形成实验观察其对胶质瘤细胞U87增殖的作用。结果分别生物信息学分析TCGA数据库和采用qPCR、Westernblot和免疫组织化学检测临床胶质瘤标本，可见胶质瘤E2F8的表达水平（-1．54±0．68）显著高于正常脑组织（-3．66±0．38），Kaplan-Meier分析表明E2F8高表达的胶质瘤患者平均生存时间为22．1个月，低表达患者为37．9个月，差异有统计学意义（P=0．025）。进一步采用siRNA沉默U87中E2F8的表达，分别采用CCK-8和集落形成检测，E2F8沉默后，U87-E2F8-siRNA组的吸光度值和集落形成率均显著低于U87-siRNA-NC组（P=0．005）。结论E2F8在胶质瘤中存在显著的高表达现象，可促进胶质瘤细胞的增殖，显著缩短患者生存时间。Objective To observe the expression of E2F8 in glioma tissues and its effect on proliferation of glioma cells. Methods Bioinformatics analysis of the cancer genome atlas （TCGA） was used to detect the expression of E2F8 in different grades of glioma. 105 cases of glioma and 12 cases of normal brain tissues were collected in our hospital. Western blotting, quantitative polymerase chain reaction （qPCR） and immunohistochemistry were performed to detect the expression of E2F8 and analyze the prog- nostic significance. The cell counting kit - 8 （ CCK - 8 ） and colony formation assays were used to detect the proliferation of U87 cells by small interfering RNA （siRNA） silencing E2FS. Results Bioinformaties analysis of TCGA database and qPCR, Western blotting and immunohistochemistry showed that the expression level of E2F8 in glioma （ - 1.54 ± 0. 68 ） was significantly higher than that in normal brain tissues （ - 3.66 ±0.38 ）. Kaplan - Meier analysis revealed that the average survival time of patients with high expression of E2F8 glioma was 22. 1 months, and that in those with low expression was 37.9 months. The difference was statistically significant （ P =0.025 ）. After E2F8 silencing, the absorbance and colony formation rate of U87 - E2F8 - siRNA group were significantly lower than those of U87 - siRNA - NC group （ P = 0. 005 ）. Conclusion E2F8 is significantly up - regulated in glioma, which can promote the proliferation of glioma cells and significantly shorten the survival time of the patients.

关 键 词：胶质瘤 E2F8 增殖 预后 

分 类 号：R730.5[医药卫生—肿瘤]