人脑胶质瘤培养组织对化疗药物的敏感性  

In vitro chemosensitivity test of glioma cells and ultrastructural changes in tissue culture

作　　者：徐立新[1] 张伟[2] 师忠芳[1] 董丽萍[1] 闫旭[1] 袁芳[1] XU Lixin;ZHANG Wei;SHI Zhongfang;DONG Liping;YAN Xu;and YUAN Fang(Department of Pathophysiology,Beijing Neurosurgical Institute,Beijing Tiantan Hospital.Capital Medical Unirersity,Beijing 100050,China;Department of Neurosur-gery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学北京市神经外科研究所病理生理室首都医科大学附属北京天坛医院,100050 [2]首都医科大学附属北京天坛医院神经外科,100050

出　　处：《武警医学》2018年第7期660-662,666,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：北京市自然科学基金项目(7102028);北京市自然科学基金项目(7152027)

摘　　要：目的观察培养的人脑胶质瘤组织对临床常用化疗药物的敏感性,为胶质瘤的个体化治疗提供实验依据。方法利用112例脑胶质瘤标本进行组织块体外培养,观察其对临床脑胶质瘤治疗常用药物尼莫司汀(ACNU)、顺铂(DDP)、替莫唑胺(TMZ)、长春新碱(VCR)、替尼泊苷(VM26)及组合用药DDP+VM26的敏感性,比较化疗药物体外抗肿瘤作用的差异;分析化疗药物敏感性与患者病理分级的相关性;应用透射电镜观察化疗药物作用前后肿瘤组织超微结构的变化。结果人脑胶质瘤培养组织对化疗药物的敏感性依次为DDP+VM26、VM26、TMZ、VCR、DDP和ACNU;仅组合用药DDP+VM26的敏感性与患者病理分级有关(χ2=4.447,P=0.035);透射电镜观察发现化疗药物作用后肿瘤细胞呈凋亡中晚期改变及坏死的变化。结论不同人脑胶质瘤培养组织对化疗药物的敏感性存在差异,引起肿瘤细胞凋亡或坏死可能是化疗药物发挥作用的机制之一。Objective To select chemotherapeutic agents based on in vitro chemosensitivity test of glioma in tissue culture in order to provide experimental data for individualized treatment of glioma. Methods One hundred and twelve specimens resected from brain glioma were used to evaluate the in vitro chemosensitivity of glioma with tissue culture. Six chemotherapeutic agent groups against brain cancer were designed,which were nimustine（ ACNU）,cisplatin（ DDP）,temozolomide（ TMZ）,vincristine（ VCR）,teniposide（ VM26） and DDP ＋ VM26. The ultrastructural changes of brain glioma after exposure to chemotherapeutic agents were observed under an electron microscope. Furthermore,the correlations between drug chemosensitivity and patients＇ pathological grade were analyzed. Results The chemosensitivity of DDP ＋ VM26 group and VM26 group was significantly higher than that of the other four groups（ P 0. 003）. Under an electron microscope,the glioma cells showed apoptosis and necrosis changes after exposure to chemotherapeutic agents. The chemosensitivity of DDP ＋ VM26 group was correlated with the pathological grade of patients（ χ2= 4. 447,P =0. 035）. Conclusions The sensitivity of patients with different glioma to chemotherapeutic drugs is different,and the apoptosis or necrosis of tumor cells may be one of the mechanisms of chemotherapeutic drugs.

关 键 词：胶质瘤 体外化疗药物敏感性试验 组织块培养法 电镜 

分 类 号：R730.53[医药卫生—肿瘤]