TNF-α在肌萎缩侧索硬化症小鼠模型脊髓中的变化  

Change of TNF-α in the spinal cord of SOD1-G93A transgenic mice

作　　者：韩笑峰 齐玉英 王媛 马文彬 王雪贞 HAN Xiaofeng ,QI Yuying ,WANG Yuan ,MA Wenbin, WANG Xuezhen （Department of Neurology, Binzhou Medical University Hospital, Binzhou 256603, P. R. China）

机构地区：滨州医学院附属医院神经内科,滨州256603

出　　处：《滨州医学院学报》2018年第5期343-345,353,共4页Journal of Binzhou Medical University

基　　金：山东省高校科技计划（J11LF57）;滨州市科技发展计划（2011ZC0905）

摘　　要：目的观察肿瘤坏死因子α（TNF-α）在SOD1-G93A转基因小鼠不同时期腰髓、胸髓中的变化，探讨TNF-α与肌萎缩侧索硬化症（ALS）小鼠模型型病进展的关系。方法采用酶联免疫吸附实验（ELISA）、RTPCR方法，观察不同疾病时期SOD1小鼠胸髓、腰髓中TNF-α水平及mRNA变化。结果SOD1小鼠在症状早期即有TNF-α分泌增加，随病情进展TNF-α水平增加。结论随病情进展TNF-α分泌水平增加，TNF-α介导的神经系统嵌症在ALS发病机制中起比较关键的作用。Objective To observe the change of TNF- α in the lumbar and thoracic spinal cord of SOD1-G93A transgenic mice at different stages and discuss the relationship between and progression of amyotrophic lateral sclerosis （ALS）. Methods The level of TNF-α and TNF-α mRNA was analyzed by ELISA and RT PCR at presymptomatic, symptomatic and end stages of SOD1-G93A transgenic mice. Results Increasing TNF-α and TNF-α mRNA appeared at presymptomatic stage in the lumbar and thoracic spinal cord of SOD1-G93A transgenic mice,and became obvious at symptomatic stage and reached the maximum at end stage. Conclusion TNF-α proliferates markedly with the progression symptom of SOD1 G93A transgenic mice. TNF-α me diated neuroinfalammation, play an important role in the pathophysiology of ALS.

关 键 词：肌萎缩侧索硬化症 肿瘤坏死因子 腰髓 胸髓 

分 类 号：R746.4[医药卫生—神经病学与精神病学;医药卫生—临床医学]