miR-30c对胃癌细胞MGC803增殖和侵袭能力的影响  

Effect of miR-30c on Invasion and Migration of Human Gastric Cancer MGC803 Cells

作　　者：霍小蕾[1] 陈晓霞[2] 刘益萍[1] 张毅强[1] Huo Xiaolei;Cheng Xiaoxia;Liu Yiping(Changzhi Medical Colleag)

机构地区：[1]长治医学院,046000 [2]三门峡市中心医院

出　　处：《长治医学院学报》2018年第5期330-333,337,共5页Journal of Changzhi Medical College

基　　金：长治医学院普及项目(QDZ201502G4)

摘　　要：目的:探讨miR-30c在胃癌中的表达及其对胃癌细胞MGC803增殖和侵袭等恶性生物学行为的影响。方法:采用逆转录定量PCR(RT-qPCR)法检测胃癌和癌旁组织中miR-30c的表达;将胃癌细胞MGC803进行转染,其中转染组细胞转染miR-30c-mimic,阴性对照组转染NC-mimic,正常对照组细胞不做任何处理,采用RT-qPCR法检测miR-30cmRNA的表达情况以判断转染效率,采用CCK8法测定细胞增殖情况,采用细胞划痕愈合试验和Transwell侵袭试验测定细胞迁移和侵袭能力。结果:与癌旁组织相比,在胃癌组织中miR-30c的表达量显著降低(P<0.05);转染组MGC803细胞miR-30cmRNA的表达量较正常对照组和阴性对照组显著增高(P<0.05),其细胞增殖率显著降低(P<0.05);细胞划痕距离显著升高(P<0.05),且细胞穿膜数量显著降低(P<0.05)。结论:miR-30c在胃癌组织中异常低表达,胃癌细胞MGC803中过表达miR-30c可抑制胃癌细胞的增殖、迁移和侵袭。Objective： To investigate the expression of miR-30c in gastric cancer tissues, and its effect on the malignant biological behaviors such as proliferation and invasion of gastric cancer cell line MGC803. Methods： The expression of miR-30c in gastric cancer and paracancerous tissues was detected by reverse transcription quantitative PCR （RT- qPCR）. The gastric cancer cells MGC803 was transfected, and the transfected group was transfected with miR-30c-mimic, the negative control group was transfected with NC-mimic, and the normal control cells were not treated. The expression of miR-30c mRNA was detected by RT-qPCR to determine the transfection efficiency. Cell proliferation was measured by CCK8 method. Cell migration and invasion ability were determined by cell scratch healing assay and Transwell invasion assay. Results： Compared with paracancerous tissues, the expression of miR-30c was significantly decreased in gastric cancer tissues （P〈0.05）. The expression level of miR-30c mRNA in the transfected group was significantly higher than that in the normal control group and the negative control group（P〈0.05） , and the cell proliferation rate was significantly decreased（P 〈0.05）. The cell scratch distance was significantly increased（P〈0.05）, and the number of cell transmembrane was significantly decreased （P〈0.05）. Conclusion： miR-30c is abnormally low in gastric cancer tissues and overexpression of miR-30c in gastric cancer cells MGC803 can inhibit the proliferation, migration and invasion of gastric cancer cells.

关 键 词：胃癌 miR-30c 增殖 细胞迁移 细胞侵袭 

分 类 号：R735.2[医药卫生—肿瘤]