α-硫辛酸对果糖诱导的实验性白内障大鼠的氧化应激、晶状体蛋白以及Ca^(2+) ATPase活性的影响  

Effects of ɑ-lipoic acid on oxidative stress,lens protein and Ca^(2+) ATPase activity in fructose induced experimental cataract

作　　者：曹娟[1] 马波[1] 吕伯昌[1] CAO Juan; MA Bo; LV Bo-chang(Department of Ophthalmology, Xi＇an NO.4 Hospital, Xi＇an Shaanxi 710004, China)

机构地区：[1]西安市第四医院眼科,陕西西安710004

出　　处：《临床和实验医学杂志》2018年第21期2249-2252,共4页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:81273902)

摘　　要：目的探讨α-硫辛酸对果糖诱导的实验性白内障的氧化应激、晶状体蛋白以及Ca^(2+) ATPase活性的影响。方法选择健康SD大鼠30只,20只大鼠通过果糖诱导建立实验性白内障模型(模型组);另外10只大鼠为空白组,不建立白内障模型。模型组中分为治疗组10只大鼠与对照组10只大鼠,治疗组在粉末状的饲料中添加0. 3%α-硫辛酸进行饲养,对照组进行常规饲养。TUNEL法检测晶体上皮细胞凋亡,酶联免疫法检测氧化应激指标,分光光度法测定晶状体蛋白活性,荧光法测定Ca^(2+) ATPase活性。结果模型组泪液中的SOD值高于空白组,MDA值低于空白组;治疗组的泪液中的SOD值低于对照组,MDA值高于对照组,差异均有统计学意义(P <0. 05)。模型组的晶体上皮细胞凋亡率、晶状体蛋白活性、Ca^(2+) ATPase活性高于空白组,治疗组晶体上皮细胞凋亡率、晶状体蛋白活性、Ca^(2+) ATPase活性低于对照组,差异均有统计学意义(P <0. 05)。结论α-硫辛酸在果糖诱导的实验性白内障大鼠中能调节机体氧化应激平衡,降低晶状体蛋白以及Ca^(2+) ATPase活性,可维持晶状体透明性,从而发挥治疗效果。Objective To investigate the effects of ɑ-lipoic acid on oxidative stress, lens protein and Ca 2＋ ATPase activity in fructose induced experimental cataract. Methods 30 healthy SD rats were selected and 20 rats were induced by fructose induction to establish experimental cataract model （model group）; the other 10 rats were the blank group that the cataract model were not established. The model group were divided into 10 rats in the treatment group and 10 rats in the control group, the treatment group were fed with 0.3% α-lipoic acid in the powder feed, and the control group were fed with conventional feeding. The apoptosis of the lens epithelial cells were detected by TUNEL method, the index of oxidative stress were detected by ELISA, the activity of lens protein were measured by spectrophotometric method and the activity of Ca 2＋ ATPase were measured by fluorescence. Results The SOD values of the tear in the model group were higher than those in the blank group, and the MDA values were lower than those in the blank group, and the SOD values in the tear of the treatment group were lower than those of the control group, and the MDA values were higher than those of the control group, and the differences were statistically significant （ P 〈0.05）. The rate of apoptosis, the activity of lens protein and the activity of Ca 2＋ ATPase in the model group were higher than those in the blank group, and the treatment group were lower than those of the control group, and the differences were statistically significant （ P 〈0.05）. Conclusion The application of ɑ-lipoic acid in fructose induced experimental cataract can regulate the oxidative stress balance of the body, reduce the activity of lens protein and Ca 2＋ ATPase, and maintain the transparency of the lens, thus plays the roles of the therapeutic effect.

关 键 词：大鼠 白内障 Α-硫辛酸 氧化应激 晶状体蛋白 CA^(2+) ATPase 

分 类 号：R776.1[医药卫生—眼科]