骨髓间充质干细胞治疗小鼠放射性唾液腺损伤的疗效分析  

The mechanism of bone marrow mesenchymal stem cells in the treatment of radiation induced salivary gland injury in mice

作　　者：程钢[1] 黄邓高[1] 梁颖[1] CHENG Gang;HUANG Deng-gao;LIANG Ying(Department of Tumor Radiotherapy,Haikou People＇s Hospital,Haikou Hainan 570208,China)

机构地区：[1]海口市人民医院肿瘤放疗科,海南海口570208

出　　处：《临床和实验医学杂志》2018年第22期2360-2363,共4页Journal of Clinical and Experimental Medicine

基　　金：海南省卫生厅科研基金项目(编号:20168315)

摘　　要：目的探讨骨髓间充质干细胞(BMSCs)治疗放射性唾液腺损伤的效果。方法选取6周龄C57小鼠150只,随机将小鼠分为正常对照组、阴性对照组及实验组,每组50只。阴性对照组和实验组分别注射生理盐水和微小唾液腺细胞小滴,同时阴性对照组和实验组再随机分为5个亚组(A、B、C、D、E亚组),分别给予12 Gy、15 Gy、18 Gy、21 Gy和24 Gy照射剂量。采用HE染色和透射电镜观察颌下腺组织结构,检测颌下腺组织重量以及小鼠唾液流量率和唾液淀粉酶。结果阴性对照组和实验组各亚组颌下腺组织腺泡细胞结构均明显受损,但实验组各亚组损伤情况轻于阴性对照组各亚组;实验组颌下腺重量为(137. 22±20. 15) mg,明显高于阴性对照组(P <0. 05),且实验组各亚组颌下腺重量[A、B、C、D、E亚组:(150. 03±17. 11) mg、(142. 06±15. 43) mg、(132. 03±17. 04) mg、(124. 06±18. 01) mg、(114. 31±19. 06) mg]均明显高于阴性对照组各亚组(P <0. 05);实验组唾液流量率为(71. 16±30. 14)μl/min,明显高于阴性对照组(P <0. 05),而唾液淀粉酶为(751. 07±79. 23)μ/dl,明显低于阴性对照组(P <0. 05);实验组各亚组唾液流量率[A、B、C、D、E亚组:(82. 24±12. 03)μl/min、(73. 55±11. 12)μl/min、(62. 20±10. 43)μl/min、(54. 03±12. 22)μl/min、(43. 31±11. 10)μl/min]均明显高于阴性对照组各亚组(P <0. 05),而唾液淀粉酶[A、B、C、D、E亚组:(651. 44±80. 14)μ/dl、(702. 24±68. 84)μ/dl、(750. 54±72. 03)μ/dl、(803. 15±80. 22)μ/dl、(845. 20±77. 46)μ/dl]明显低于阴性对照组各亚组(P <0. 05)。结论骨髓间充质干细胞对小鼠放射性唾液腺损伤有一定保护作用,可促进唾液腺细胞结构和功能的恢复。Objective To investigate the role of bone marrow mesenchymal stem cells （BMSCs） in radiation - induced salivary gland injury. Methods 150 C57 mice aged 6 weeks were randomly divided into normal control group, negative control group and experimental group, 50 mice in each group. The negative control group and experimental group were injected with saline and tiny droplets of cells of salivary gland, at the same time, the negative control group and the experimental group were randomly divided into 5 subgroups （A, B, C, D and E subgroups）, were given respectively the radiationdoses of 12 Gy, 15 Gy, 18 Gy, 21 Gy and 24 Gy. HE staining and transmission electron microscopy were used to observe the histological structure of the submandibular gland, the weight of the submandibular gland tissue, the rate of saliva flow and the amylase in the saliva of mice were detected. Results In tbe negative control group and tbe experimental group, tbe submandibular gland tissue aeinar cell structure were significantly damaged, but the injury of each subgroup in the experimental group was less than that in the negative control group. The experimental group of submandibular gland weight was （ 137.22 ± 20.15） mg, significantly higher than that in the negative control group （ P 〈 0.05） , and experimental group each subgroups submandibular gland weight [ A, B, C D and E sub groups： （150.03 ± 17.11 ）mg, （142.06 ± 15.43 ）mg, （ 132.03 ± 17.04 ）mg, （ 124.06 ±18.01 ） mg, （ 114.31± 19.06 ）mg ] were significantly higher than those of negative control groupsubgroups （ P 〈 0.05）. The salivary flow rate of experimental group was （71.16 ± 30.14）μl/min, significantly higher than that of control group （ P 〈 0.05 ） , and salivary amylase was （751.07 ± 79.23 ）μ/dl, significantly lower than that of the negative control group （ P 〈 0.05 ）. The salivary flow rate of experimental group [ A, B, C, D, E subgroups ： （82.24± 12.03 ） μl/min, （73.55 ± 11.12） μl/min, （62.2

关 键 词：小鼠 放射性唾液腺损伤 骨髓间充质干细胞 颌下腺 

分 类 号：R730.55[医药卫生—肿瘤]