过表达RFX1对胶质瘤细胞增殖和侵袭的影响  

Effects of RFX1 over-expression on proliferation and invasion of glioma cells

作　　者：程凯[1] 张亚萍 曹丽 张晓延[3] CHENG Kai;ZHANG Yaping;CAO Li;ZHANG Xiaoyan(Department of Biochemistry;Science and Technology Center;Department of Pathogenic Biology,Fenyang College of Shanxi Medical University,Fenyang 032200,Shanxi,China)

机构地区：[1]山西医科大学汾阳学院生物化学教研室,山西汾阳032200 [2]山西医科大学汾阳学院科技中心,山西汾阳032200 [3]山西医科大学汾阳学院病原生物学教研室,山西汾阳032200

出　　处：《中国肿瘤生物治疗杂志》2018年第10期1021-1025,共5页Chinese Journal of Cancer Biotherapy

基　　金：山西医科大学汾阳学院科技发展基金资助项目(No.2016805).

摘　　要：目的:探讨过表达调节因子X1基因(regulatory factor X1,RFX1)对神经胶质瘤细胞株F98细胞增殖和侵袭能力的影响及其作用机制。方法:用慢病毒转染法将RFX1转染到F98细胞,构建过表达RFX1的F98细胞株(F98-RFX1组),同时设立空质粒对照组(F98-Vector组)和正常培养组(F98组)。用计数法观察过表达RFX1对各组F98细胞增殖的影响,用AnnexinV/PI染色法流式细胞术检测过表达RFX1对F98细胞凋亡的影响,用Transwell小室法检测过表达RFX1对F98细胞侵袭能力的影响。结果:成功建立过表达RFX1胶质瘤F98细胞株。过表达RFX1组胶质瘤F98细胞的增殖能力显著低于F98组[48 h:(12.08±2.17)×10~4/ml vs(23.67±4.51)×10~4/ml,P<0.05]和F98-Vector组[96 h:(8.17±0.31)×10~4/ml vs(18.58±1.18)×10~4/ml;P<0.05];过表达RFX1组细胞的凋亡水平明显上升[(21.89±2.33)%vs(3.38±1.39)%、(10.42±1.83)%,P<0.05];过表达RFX1组细胞侵袭能力显著下降[(33.3±7.99)vs(56.5±13.9)、(60.6±11.8)个,P<0.01]。结论:RFX1可以调控增殖和侵袭相关基因的表达,进而抑制胶质瘤细胞的增殖和侵袭能力、促进细胞凋亡。Objective： To validate the effect and the possible mechanism of human regulatory factor X1 （RFX1） over-expression on the proliferation and invasion of glioma F98 cells. Methods： RFX1-overexpressed F98 cells （F98-RFX1 group） were constructed by lentivirus transfection, a control group （F98-Vector group） and normal group （F98 group） were established. The effect of RFX1 over-expression on F98 cell proliferation was observed with counting method, cell apoptosis was determined by AnnexinV-PI staining, and the cell invasion was observed with Transwell method. Results： F98 cell line over-expressing RFX1 was successfully established. The proliferation capacity of F98-RFX1 group was significantly lower than that of F98 group （48 h： [12.08±2.17]×10^4/ml vs [23.67±4.51]×10^4/ml, P〈0.05] and F98-Vector group （96 h： [8.17±0.31]×10^4 /ml vs [18.58±1.18]×10^4 /ml, P〈0.05）; The apoptosis level of cells in F98-RFX1 group was significantly increased （[21.89±2.33]% vs [3.38±1.39]%, [10.42±1.83]%, P〈0.05]; The invasiveness of cells in F98-RFX1 group was significantly reduced （[33.3±7.99] vs [56.5±13.9], [60.6±11.8], P〈0.01）. Conclusion： RFX1 can regulate the expression of genes related with proliferation and invasion, thereby inhibiting the proliferation and invasion ability of glioma cells and promote cell apoptosis.

关 键 词：恶性胶质瘤 F98细胞 调节因子X1(RFX1) 增殖 侵袭 凋亡 

分 类 号：R737.25[医药卫生—肿瘤] R730.54[医药卫生—临床医学]