miR-139在肺鳞癌组织中的表达及临床意义  

Expression of miR-139 in Squamous Cell Lung Cancer and Its Clinical Significance

作　　者：阮红莲 叶芷璇 杨磊 王书强 施昌宏 Ruan Honglian;Ye Zhixuan;Yang Lei;Wang Shuqiang;Shi Changhong(Department of Applied Statistics,School of Public Health,Guangzhou Medical University,Guangzhou 511436,Guangdong,China;Department of Preventive Medicine,School of Public Health,Guangzhou Medical University,Guangzhou 511436,Guangdong,China;Institute of Advanced Computing and Digital Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,Guangdong,China)

机构地区：[1]广州医科大学公共卫生学院应用统计系,广州511436 [2]广州医科大学公共卫生学院预防医学系,广州511436 [3]中国科学院深圳先进研究院高性能计算技术研究中心,广东深圳518055

出　　处：《肿瘤预防与治疗》2018年第5期313-317,共5页Journal of Cancer Control And Treatment

基　　金：广东省自然科学基金(编号:2014A030310181);广东省医学科学研究技术基金(编号:A2015389)

摘　　要：目的:分析miR-139在肺鳞癌组织中的表达及临床意义,预测与肺鳞癌预后相关的miR-139的靶基因。方法:从癌症基因组图谱(TCGA)数据库收集肺鳞癌数据集,下载患者临床特征、基因表达谱及预后随访信息。分析miR-139表达与肺鳞癌临床特征的相关性及对预后的影响。利用生物信息学方法预测miR-139的靶基因并进行生存分析。结果:在45对肺鳞癌和配对癌旁正常组织中,癌组织miR-139表达低于正常组织,且差异具有统计学意义(P <0. 001)。在Ⅰ/Ⅱ期肺鳞癌组织中,miR-139相对高表达组的总生存情况不如miR-139相对低表达组,其中位生存期分别为1713日和3253日,差异具有统计学意义(P <0. 001)。多因素Cox回归分析发现,miR-139是肺鳞癌的独立预后因子,高表达患者的死亡风险是低表达患者的2. 768倍(P <0. 001)。结论:在肺鳞癌组织中,miR-139相对高表达是一种预后不良因素,经独立验证之后有望作为预测肺鳞癌患者预后的生物标志物。Objective： To investigate the expression of miR-139 in squamous cell lung cancer（SCLC）, and to predict possible target genes of miR-139 related to the prognosis of SCLC. Methogs： The gene expression profile in SCLC and their clinical data were downloaded from The Cancer Genome Atlas （TCGA）. The prognostic value of miR-139 expression and the relationship between miR-138 expression and cnlinian fentures of SCLC ware analyzed. The target genes of miR-139 were predicted by online bioinformatic tools, and their relationship with prognosis were analyzed. Results： The expression of miR-139 in cancer tissue was significantly lower than that in the paired adjacent nontumorous tissue. In stage Ⅰ and Ⅱ cancer tissue, relatively higher expression of miR-139 was associated with inferior overall survival （OS）. Furthermore, miR-139 was proved to be an independent negative prognosis factor for OS in multivariate survival analysis. Conclusion： miR-139 could be used as a potential prognostic marker in SCLC.

关 键 词：肺鳞癌 TCGA miR-139 预后 

分 类 号：R734.2[医药卫生—肿瘤]