氢气对心搏骤停兔心肌糖代谢的PET显像的影响  

Application of positron emission tomography in detection of myocardial glucose metabolism in rabbit cardiac arrest models

作　　者：姜广军 黄国庆[2] 李湘民[2] 姚政彬 唐永祥[3] 艾宇航[1] JIANG Guangjun;HUANG Guoqing;LI Xiangmin;YAO Zhengbin;TANG Yongxiang;AI Yuhang(Department of Intensive Care Unit;Department of Emergency;Department of PET Center,Xiangya Hospital,Central South University,Changsha 410008,China)

机构地区：[1]中南大学湘雅医院重症医学科,长沙410008 [2]中南大学湘雅医院急诊科,长沙410008 [3]中南大学湘雅医院PET中心,长沙410008

出　　处：《中南大学学报（医学版）》2018年第10期1112-1117,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81401024);北京协和医学基金会“睿E(睿意)急诊医学研究专项基金”(R201S00S)。

摘　　要：目的:通过18F-氟-2-脱氧葡萄糖(18F-fluroro-2-deoxyglucose,18F-FDG)正电子发射断层成像(positron emission tomography,PET)了解心搏骤停兔心肌糖代谢的变化规律及氢气干预对其影响。方法:雄性新西兰大白兔15只,分为3组:氢气组6只,对照组6只,假手术组3只。通过静脉注射氯化钾诱导心搏骤停兔模型,在心搏骤停5 min后开始行心肺复苏。氢气组与对照组开始复苏时分别予以4%氢气+96%氧气和纯氧吸入,持续30 min,假手术组麻醉、气管插管后予以肾上腺素注射+氯化钾缓慢注射,但未诱导心搏骤停。记录各组动物基础及复苏后30 min生命体征和复苏相关参数。3组均于基础状态及复苏后2和24 h利用PET测定左心室心肌18F-FDG最大标准化摄取值(maximum standardized uptake value,SUVmax)。结果:3组基础体重、生命体征及行PET检查前血糖水平差异均无统计学意义(均P>0.05)。假手术组3次左心室18F-FDG SUVmax值无显著变化。氢气组及对照组复苏后2 h左心室18F-FDG SUVmax较基础值明显升高(分别为1.89±0.47 vs 3.47±1.24及1.90±0.36 vs 4.26±0.80,P<0.01),与对照组相比,氢气组升高幅度较低,24 h后均明显回降(氢气组2.02±0.64,对照组2.07±0.61),与基础值差异无统计学意义(P>0.05)。结论:心搏骤停复苏后兔心肌糖代谢存在明显增强过程,氢气干预可降低糖代谢增强的程度。Objective： To investigate the changes of myocardial glucose metabolism in rabbit cardiac arrest models and the eff ect of hydrogen intervention by ^18F-fl uroro-2-deoxyglucose （^18F-FDG） positron emission tomography （PET） imaging. Methods： Fifteen male New Zealand white rabbits were randomly divided into a hydrogen group （n=6）, a control group （n=6） and a sham group （n=3）. Cardiac arrest （CA） was induced by intravenous injection of potassium chloride. Conventional cardiopulmonary resuscitation （CPR） was initiated after five-minutes CA. The hydrogen group and the control group were mechanically ventilated into mixed gas with 4% hydrogen＋96% oxygen and pure oxygen, respectively, for 30 minutes after CPR. Rats in the sham group was performed the same surgical procedure and was injected adrenaline and potassium chloride but did not induce CA. The vital signs at basic state and 30 min after return of spontaneous circulation （ROSC） were recorded in each group. The parameters of CPR were recorded in two CA groups. Myocardial glucose metabolism was assessed by positron emission tomography （PET） at basic state, 2 h and 24 h after ROSC. The maximum standardized uptake value （SUVmax） of ^18F-FDG was measured. Results： There were no significant differences in the basal body weight and vital signs among the three groups. There was no significant difference in the blood glucose level before PET examination. The ^18F-FDG SUVmax in the sham group at three time points was not significantly changed. In the hydrogen group and the control group, the ^18F-FDG SUVmax at 2 h after ROSC were significantly higher than the basic level （1.89±0.47 vs 3.47±1.24 and 1.90±0.36 vs 4.26±0.80, respectively）. Compared with the control group, the ^18F-FDG SUVmax in the hydrogen group was lower at the point at 2 h after ROSC. The ^18F-FDG SUVmax in the 2 CA group were down to the basic level at 24 h after ROSC （hydrogen group 2.02±0.64, control group 2.07±0.61）. Conclusion： Myocardial g

关 键 词：氢气 心搏骤停 心肺复苏 葡萄糖代谢 正电子发射断层成像 兔 

分 类 号：R541.78[医药卫生—心血管疾病]