窖水中微生物降解污染物的关键细菌  

Key Bacteria for the Microbial Degradation of Pollutants in Cellar Water

作　　者：杨浩[1,2] 杨晓妮[3] 张国珍[1,2] 王宝山[1,2] 张翔[1] 李健 YANG Hao;YANG Xiao-ni;ZHANG Guo-zhen;WANG Bao-shan;ZHANG Xiang;LI Jian(School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Engineering Research Center for Cold and Arid Regions Water Resource Comprehensive Utilization,Ministry of Education,Lanzhou 730070,China;College of Natural Resources and Environment,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]兰州交通大学环境与市政工程学院,兰州730070 [2]寒旱地区水资源综合利用教育部工程研究中心,兰州730070 [3]甘肃农业大学资源与环境学院,兰州730070

出　　处：《环境科学》2018年第10期4766-4777,共12页Environmental Science

基　　金：国家自然科学基金项目(51768031;51368029)

摘　　要：为探究以氮、磷及有机物污染为主要特征的、窖水中参与污染物降解的关键细菌及它们之间潜在的相关关系,基于16S rRNA的微生物组学截面数据,分析了窖水中细菌群落结构与功能及其与水质因子间的相关性,并通过微生物物种的同现或相关种间作用推断模型,构建了7个微生物菌属间的共现性关联网络.结果表明,窖水中存在具有相对特定生态功能的细菌,进行着诸多活跃的与新陈代谢功能基因相关的代谢活动;窖水微生物类群共现性关联网络中大部分节点的菌属营互利共生类型的生态关系;Lacibacter、Arthrobacter、Candidatus Protochlamydia、Methylocaldum、Sulfuritalea、Mycobacterium、Aquirestis、Rhodobacter、Methylotenera等菌属拥有较高的点度中心度;较强的互作关系发生在Sulfuritalea-Rhodobacter、Azospirillum-Rhodobacter、Methylocaldum-Rhodobacter、Arthrobacter-Rhodobacter、Rhodoplanes-Rhodobacter、Candidatus Protochlamydia-Rhodobacter、Methylotenera-Rhodobacter、Rhodobacter-Aquirestis、Mycobacterium-Rhodobacter、PlanctomycesCandidatus Solibacter、Planctomyces-Legionella、Hymenobacter-Adhaeribacter、Luteolibacter-Crenothrix之间.综合分析节点微生物相关性、点度中心度及菌属间的互作强度,认为Rhodobacter、Methylocaldum、Methylotenera、Acinetobacter、Novosphingobium、Planctomyces、Hymenobacter、Luteolibacter为参与窖水污染物微生物降解的关键细菌,Rhodobacter为关键细菌的代表属.研究结果加深了对窖水中污染物微生物降解机制的认识.The study aimed to identify the key bacteria and the potential interactions among these bacteria during the degradation of pollutants in cellar water,The main pollution characteristics were nitrogen,phosphorus and organic pollution. The structure and function of the bacterial community and its correlation with water quality variables were analyzed. A network of seven associations of microbial co-occurrence was set up,based on 16 S rRNA and the model for inferring co-occurrence or interspecific interactions of microbial species. This showed that there were a large number of microorganisms with relatively specific ecological functions in the cellar water,and that many metabolic activities were involved. The ecological relationships of most bacteria in the association network were a form of mutualism. The most prominent genera included Lacibacter,Arthrobacter,Candidatus Protochlamydia,Methylocaldum,Sulfuritalea,Mycobacterium,Aquirestis,Rhodobacter,and,Methylotenera. The strong associations were observed between following bacteria： Sulfuritalea-Rhodobacter, Azospirillum-Rhodobacter, Methylocaldum-Rhodobacter, Arthrobacter-Rhodobacter, RhodoplanesRhodobacter, Candidatus Protochlamydia-Rhodobacter, Methylotenera-Rhodobacter, Rhodobacter-Aquirestis, MycobacteriumRhodobacter,Planctomyces-Candidatus Solibacter,Planctomyces-Legionella,Hymenobacter-Adhaeribacter,and Luteolibacter-Crenothrix.It was considered that Rhodobacter,Methylocaldum,Methylotenera,Acinetobacter,Novosphingobium,Planctomyces,Hymenobacter,and Luteolibacter were the key bacteria involved in microbial degradation of cellar water pollutants, and Rhodobacter was the representative genus of the key bacteria. The authors concluded that the research results improved understanding of the microbial degradation mechanism of pollutants in cellar water.

关 键 词：集雨窖水 细菌群落组成 群落基因功能 微生物共现性关联网络 关键细菌 

分 类 号：X172[环境科学与工程—环境科学]