Caspase-9(-Ex5+32 G/A)基因多态性与其在退变腰椎间盘髓核组织表达的相关性分析  

Caspase-9(-Ex5 + 32 G/A ) gene polymorphism is associated with caspase-9 expression in degenerative nucleus pulposus of lumbar intervertebral disc

作　　者：靳占奎[1] 刘振国[2] 徐翠香[3] 杨江存[4] 徐洪海[1] 常彦海[1] 董向辉[1] 吴学元[1] 孙正明[1] JIN Zhankui;LIU Zhenguo;XU Cuixiang;YANG Jiangcun;XU Honghai;CHANG Yanhai;DONG Xianghui;WU Xueyuan;SUN Zhengming(Department of Orthopaedics;Department of Intensive Care Unit;Center of Shaanxi Provincial Clinical Laboratory;Department of Blood Transfusion,Shaanxi Provincial People＇s Hospital,Xi＇an 710068,China)

机构地区：[1]陕西省人民医院骨科,陕西西安710068 [2]陕西省人民医院重症医学科,陕西西安710068 [3]陕西省人民医院陕西省临床检验中心,陕西西安710068 [4]陕西省人民医院输血科,陕西西安710068

出　　处：《细胞与分子免疫学杂志》2018年第6期517-521,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：陕西省国际科技合作与交流计划项目(2017KW-051)

摘　　要：目的探讨胱天蛋白酶9(caspase-9)的-Ex5+32 G/A基因多态性、腰椎磁共振(MRI)椎间盘Schneiderman评分与退变的腰椎间盘髓核组织caspase-9表达之间的关系。方法获取105例腰椎间盘突出症患者的外周静脉血及突出的髓核组织;提取全基因组DNA,采用基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF-MS)分析全部DNA标本的caspase-9(-Ex5+32 G/A)基因多态性,免疫组织化学SP染色法检测髓核组织中caspase-9的表达。t检验分析各基因型与椎间盘髓核caspase-9表达之间的差异,用Pearson相关性分析评价髓核组织caspase-9表达和腰椎间盘退变的MRI评分的关联性。结果 MRI分析显示,携带AA基因型患者的腰椎间盘MRI评分最高,但AA、GA、GG三组基因型患者的腰椎间盘MRI评分差异不明显;与GG基因型携带者相比,AA基因型携带者髓核组织高表达caspase-9;腰椎间盘MRI评分与退变的腰椎间盘髓核组织caspase-9表达无相关性。结论 Caspase-9的-Ex5+32 G/A基因多态性和退变腰椎间盘髓核组织caspase-9的表达有关;MRI腰椎间盘Schneiderman评分与退变的髓核组织caspase-9表达无相关性。Objective To investigate the relationship between caspase-9（-Ex5 ＋ 32 /） gene polymorphisms,Schneiderman score of magnetic resonance imaging（ MRI） in lumbar disc degeneration and the expression of caspase-9 in degenerative nucleus pulposus. Methods The peripheral venous blood and prominent nucleus pulposus were obtained from105 patients with lumbar disc herniation. Genomic DNA was extracted and analyzed by matrix-assisted laser desorptio/onization time-of-flight mass spectrometry（ MALDI-TOF-MS）. The caspase-9（-Ex5 ＋ 32 /） gene polymorphisms were analyzed in all DNA samples. The expression of caspase-9 in the tissues was detected by immunohistochemical SP staining. The t test was used to analyze the difference between the genotypes and the expression of caspase-9 in intervertebral disc nucleus.Pearson correlation analysis was performed to assess the association between caspase-9 expression in nucleus pulposus and the MRI score of lumbar disc. Results MRI analysis showed that the patients with AA genotype had the highest MRI scores,but there were no significant differences in MRI scores among patients with AA,GA,and GG genotypes. Compared with GG genotype carriers,AA genotype carriers had a statistically significant difference in the expression of caspase-9 in nucleus pulposus. There was no correlation between the MRI score of lumbar disc degeneration and the expression of caspase-9 in the degenerated nucleus pulposus. Conclusion The caspase-9（-Ex5 ＋ 32 /） gene polymorphism is associated with the expression of caspase-9 in the degenerative nucleus pulposus. However,the MRI Schneiderman score is not significantly correlated with the expression of caspase-9 in degenerative nucleus pulposus.

关 键 词：腰椎间盘突出症 退变 髓核 基因多态性 胱天蛋白酶9(caspase-9) 

分 类 号：Q344.12[生物学—遗传学] R745.42[医药卫生—神经病学与精神病学]