结直肠癌患者外周血G-MDSC和M-MDSC水平与疗效的相关性分析  

Correlation analysis between the numbers of G-MDSCs and M-MDSCs in peripheral blood decrease and therapeutic effect in patients with colorectal cancer

作　　者：王永仿 许联红[1] 恽志华 楚鹰[1] 法镇中[2] 邓建忠[3] 张颖[1] 吴佳 WANG Yongfang;XU Lianhong;YUN Zhihua;CHU Ying;FA Zhenzhong;DENG Jianzhong;ZHANG Ying;WU Jia(Department of Clinical laboratory;Department of General Surgery;Department of Oncology,Affiliated Wujin People＇s Hospital,Jiangsu University,Changzhou 213002,China;School of Medicine and Technology,Jiangsu University,Zhenjiang 212000,China)

机构地区：[1]江苏大学附属武进医院检验科,江苏常州213002 [2]江苏大学附属武进医院普外科,江苏常州213002 [3]江苏大学附属武进医院肿瘤内科,江苏常州213002 [4]江苏大学医学技术学院,江苏镇江212000

出　　处：《细胞与分子免疫学杂志》2018年第6期546-551,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：武进科技计划项目(WS2014014);江苏大学临床项目(JLY20140039)

摘　　要：目的检测结直肠癌患者外周血中髓源抑制细胞(MDSC)频数,分析MDSC变化与结直肠癌临床病理特征的关系。方法采用流式细胞术分别检测82例结直肠癌患者和30例健康志愿者外周血中粒细胞型MDSC(G-MDSC)和单核细胞型MDSC(M-MDSC)频数。使用方差分析和t检验分析结直肠癌患者外周血中MDSC频数与TNM分期、淋巴结转移、肿瘤部位、分化程度的关系以及常用治疗措施对MDSC的影响。用相关分析评价G-MDSC与M-MDSC频数之间的相关性。结果与健康志愿者相比,结直肠癌患者组外周血中G-MDSC和M-MDSC频数显著增高。TNMⅢ、Ⅳ期结直肠癌患者外周血G-MDSC与M-MDSC频数显著高于TNMⅠ、Ⅱ期患者,且发生淋巴结转移患者的G-MDSC和M-MDSC频数亦显著高于无淋巴结转移的患者。外周血G-MDSC和M-MDSC频数在不同肿瘤部位与分化程度的结直肠癌患者间无明显差异。施行根治性切除术以及经辅助化疗有效的结直肠癌患者外周血G-MDSC和M-MDSC频数显著下降。结直肠癌患者外周血G-MDSC与M-MDSC频数之间无相关性。结论结直肠癌患者外周血G-MDSC和M-MDSC频数均显著升高,并且其频数与TNM分期及淋巴结转移有关。根治性切除术及有效的辅助化疗可显著降低结直肠癌患者外周血G-MDSC和M-MDSC频数。Objective To detect the frequencies of myeloid-derived suppressor cells（ MDSCs） in the peripheral blood of patients with colorectal cancer（ CRC） in an attempt to investigate the relationship between the change of MDSC frequencies and clinicopathologic characteristics of CRC patients. Methods The frequencies of granulocytic MDSCs（ G-MDSCs） and monocytic MDSCs（ M-MDSCs） in the peripheral blood of 82 CRC patients and 30 healthy volunteers were detected by flow cytometry. ANOVA and t-test were used to explore the relationship between the frequencies of MDSCs in the peripheral blood and the TNM stages,lymph node metastasis,tumor sites,histological grade as well as common therapeutic measures of CRC patients. Correlation analysis was performed to evaluate the correlation between the frequencies of G-MDSCs and M-MDSCs. Results Compared with the 30 healthy volunteers,the frequencies of G-MDSCs and M-MDSCs in the peripheral blood of the 82 CRC patients were statistically higher. The frequencies of G-MDSCs and M-MDSCs in the peripheral blood from TNF stage Ⅲ and Ⅳ CRC patients were obviously higher than those from stage Ⅰ and Ⅱ patients,and CRC patients with lymph node metastasis had remarkably higher frequencies of G-MDSCs and M-MDSCs than the patients without lymph node metastasis. There were no statistical differences in the frequencies of G-MDSCs and M-MDSCs in the peripheral blood between CRC patients with different tumor sites as well as histological grades. Radical resection and effective adjuvantchemotherapy significantly decreased the frequencies of G-MDSCs and M-MDSCs in the peripheral blood of CRC patients.No statistical correlation was found between the frequencies of circulating G-MDSCs and M-MDSCs in CRC patients.Conclusion G-MDSC and M-MDSC frequencies in the peripheral blood of CRC patients significantly increased,and were closely associated with TNM stages and lymph node metastasis. Radical resection and effective adjuvant chemotherapy significantly reduced the frequencies of circul

关 键 词：结直肠癌 粒细胞型髓源抑制细胞 单核细胞型髓源抑制细胞 肿瘤免疫 

分 类 号：R735.37[医药卫生—肿瘤] R730.3[医药卫生—临床医学]