LncRNA AP000472.2在甲状腺乳头状癌细胞中的表达情况分析  

Expression of Long Non-coding RNA AP000472.2 in Papillary Thyroid Carcinoma

作　　者：蒋烈浩[1] 殷珂欣 谭卓[1] 黄煜庆 温庆良 朱欣[1] 葛明华[1] JIANG Lie-hao;YIN Ke-xin;TAN Zhuo;HUANG Yu-qing;WEN Qing-liang;ZHU Xin;GE Ming-hua(Zhejiang Cancer Hospital,Zhejiang Provincial Key Laboratory of Head ＆ Neck Cancer Translational Research,Hangzhou 310022,China;School of Stomatology,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江省肿瘤医院浙江省头颈肿瘤转化医院研究重点实验室,浙江杭州310022 [2]浙江中医药大学第二临床医学院,浙江杭州310053

出　　处：《中国肿瘤》2018年第10期801-806,共6页China Cancer

基　　金：国家自然科学基金青年基金(81702644);国家自然科学基金面上项目(81672642);浙江省自然科学基金面上项目(LY17H070003)

摘　　要：[目的]通过检测甲状腺乳头状癌（papillary thyroid carcinoma,PTC）及癌旁正常组织中长链非编码RNA（long non-coding RNA,lnc RNA）AP000472.2的相对表达量,分析其与PTC临床病理特征的关系。[方法]采用q RT-PCR技术检测lnc RNA AP000472.2在60例新鲜冰冻PTC组织及配对癌旁组织的表达水平,并分析其与PTC各临床因素之间的相关性。[结果 ]Lnc RNA AP000472.2在PTC肿瘤组织中表达水平明显高于癌旁正常组织,差异有统计学意义（P〈0.01）;存在包膜侵犯或淋巴结转移的甲状腺乳头状癌组织中lnc RNA AP000472.2表达水平明显高于无包膜侵犯或无淋巴结转移的甲状腺乳头状癌组织,差异具有统计学意义（P〈0.05）;而不同性别、年龄、腺内播散、砂粒体、肿瘤大小、肿瘤数量之间lnc RNA AP000472.2表达水平无明显统计学差异（P〉0.05）。[结论]Lnc RNA AP000472.2高表达可能与PTC的发生、发展存在密切联系,且在PTC包膜侵犯和颈部淋巴结转移中发挥着一定的作用。[Purpose] To investigate the expression of long-coding RNA（lnc RNA） AP000472.2 in papillary thyroid carcinoma（PTC）and its relation to clinicopathologic factors. [Methods] The expression of lnc RNA AP000472.2 was detected by RT-PCR in 60 fresh frozen PTC tissues and its matched pericancerous tissues. [Results] The expression of lnc RNA AP000472.2 significantly upregulated in PTC tissues compared with pericancerous tissues（P0.05）. The expression of lnc RNA AP000472.2 was significantly associated with capsular invasion and lymph node metastasis,while not associated with gender,age,lobe dissemination,psammoma body,tumor size and tumor number. [Conclusion] The high-expressed lnc RNA AP000472.2 may lead to the development of PTC,and involve in the capsular invasion and lymph node metastasis of papillary thyroid carcinoma.

关 键 词：甲状腺乳头状癌 长链非编码RNA AP000472.2 侵袭 转移 

分 类 号：R736.1[医药卫生—肿瘤]