低密度脂蛋白受体基因多态性与脑梗死相关性的研究  

Relation between LDLR Gene PvuII polymorphism and cerebral infarction

作　　者：邓颖[1] 于汉力[1] 卞福民[2] 薛雅丽 于敏 傅松滨[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院急诊科,150080 [2]浙江省临海市台州医院

出　　处：《中国急救医学》2002年第9期507-508,共2页Chinese Journal of Critical Care Medicine

摘　　要：目的　探讨低密度脂蛋白受体 (LDLR)基因内含子 15PvuII多态性与动脉硬化性脑梗死的相关性 ,并对其与血脂之间的关系进行分析。方法　采用聚合链反应 (PCR)和限制性片段长度多态性 (RFLP)方法 ,检测了 5 2例脑梗死患者与 6 7例健康对照组LDLR基因含有PvuII酶切位点的等位基因频率和基因型分布。结果　脑梗死患者含有PvuII酶切位点的基因频率与正常人比较差异不显著 (P >0 0 5 )。结论　脑梗死患者血浆LDL、TC、TG水平增高 ,HDL水平降低是脑梗死的危险因素 ;LDLR基因内含子 15PvuII位点不能确定为脑梗死的遗传标记。Objective The study explored the relation between the polymorphism of LDL-receptor gene intron 15 PvuⅡ, cerebral infarction and lipoprotein in plasma. Methods The frequency of PvuⅡ cutting site, allele frequency and genotype frequency were detected by polymerase chain reaction and restrictive fragment length polymorphism (PCR-RFLP) analysis. LDL-receptor gene intron 15 PvuⅡ were detected in 52 patients with cerebral infarction compared to 67 healthy control, then analyzed. Results Frequency of LDLR intron PvuⅡ cutting in patient with cerebral infarction was similar to that in healthy control. Conclusions The raising of LDL, TG, TC in plasma and lowering of HDL are risk factors of cerebral infarction. The study failed to detect to siganificant association between PvuⅡ genotype and cerebral infarction.

关 键 词：低密度脂蛋白受体 基因多态性 脑梗死 低密度脂蛋白 甘油三酯 限制性片段长度多态性 血脂 聚合酶链反应 

分 类 号：R743.33[医药卫生—神经病学与精神病学]