妊娠期糖尿病胎鼠胎盘中脂代谢相关因子的研究  

Study on Lipid Metabolism Related Factors in Placenta of Gestational Diabetes Mellitus Rats

作　　者：张青苗 宋婷 欧阳为相[1] 柴新群[2] 邓飞涛[1] ZHANG Qingmiao;SONG Ting;OUYANG Weixiang;CHAI Xinqun;DENG Feitao(Department of Obstetrics and Gynecology,Union Hospital,Huazhong University of Science and Technology,Wuhan,430022,China;Department of Hepatobiliary Surgery,Union Hospital,Huazhong University of Science and Technology,Wuhan,430022,China)

机构地区：[1]华中科技大学附属协和医院妇产科,武汉市430022 [2]华中科技大学附属协和医院肝胆外科,武汉市430022

出　　处：《医学分子生物学杂志》2018年第5期332-337,共6页Journal of Medical Molecular Biology

基　　金：国家自然科学基金（No.30971072）,湖北省自然科学基金（No.2017CFB749）

摘　　要：目的探讨妊娠糖尿病孕鼠胎盘中脂代谢相关因子雷帕霉素靶蛋白（mammaliantargetofrapamy．ein，mTOR）和脂质（Lipin）表达情况。方法妊娠大鼠分成实验组（即糖尿病组），干预组（糖尿病模型＋雷帕霉素）及对照组（柠檬酸钠缓冲液）。于孕21d取出胎盘组织，通过免疫组化技术检测各组胎盘组织中p-mTOR、Lipin的定位表达；利用Western印迹技术检测胎盘组织中磷酸化雷帕霉素靶蛋白p-mTOR、mTOR、Lipin蛋白表达水平：利用RT-PCR技术检测胎盘组织中mT0R、Lipin的mRNAs表达水平。结果实验组中胎盘组织p-mTOR及Lipin水平均高于对照组，而干预组水平介于两者之间（P〈0．05）；结论GDM孕鼠可能通过激活mTOR通路，使其过度磷酸化，提高Lipin水平，进而影响胎盘组织中脂质代谢。Objective To investigate the expression of lipid metabolism related factors （mTOR, Lipin） in placenta of gestational diabetes mellitus rats. Methods Pregnant rats were di- vided into experimental group （diabetic group）, intervention group （diabetic model ＋ rapamycin） and control group （ sodium citrate buffer） . On the 21 st day of gestation, the placenta tissues were taken out, and the expression of p-mTOR and Lipin were detected by immunohistochemistry, the expression of p-mTOR, roTOR and Lipin were detected by Western Blot, and the expression of mTOR and Lipin mRNAs were detected by RT-PCR. Results The levels of p-mTOR and Lipin in experimental group were higher than those in control group, but the levels in intervention group were between them （P 〈 0. 05 ） . Conclusion The abnormal lipid metabolism in the placenta of GDM pregnant rats may be caused by activating the mTOR pathway and increasing the level of Lip- lB.

关 键 词：妊娠期糖尿病 胎盘 MTOR LIPIN 

分 类 号：R714.256[医药卫生—妇产科学]