基于网络药理学的驴胶补血颗粒升高白细胞作用机制研究  

An exploration into mechanism of leukocyte elevation activity of Lvjiao Buxue granules based on network pharmacology

作　　者：颜磊 何小燕[1,2] 高耀 向欢[3] 徐向平 黄胜[4] 颜冬兰 秦雪梅 田俊生 YAN Lei;HE Xiao-yan;GAO Yao;XIANG Huan;XU Xiang-ping;HUANG Sheng;YAN Dong-lan;QIN Xue-mei;TIANJun-sheng(Modern Research Center for Traditional Chinese Medicine;Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM;School of Physical Education,Shanxi University,Taiyuan 030006,China;Jiuzhitang Co.Ltd.Hunan,Changsha 410205,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学地产中药功效物质研究与利用山西省重点实验室,山西太原030006 [3]山西大学体育学院,山西太原030006 [4]九芝堂股份有限公司,湖南长沙410205

出　　处：《药学学报》2018年第10期1660-1669,共10页Acta Pharmaceutica Sinica

基　　金：中国博士后科学基金面上资助项目(2016M602414);山西省科技重点研发计划(201603D3113013;201603D321077;201701D22111344);地产中药功效物质研究与利用山西省重点实验室项目(201605D111004);山西省科技创新重点团队项目(201605D131045-18)

摘　　要：构建驴胶补血颗粒活性成分-作用靶点网络及蛋白相互作用网络,对靶点涉及的功能和通路进行分析,从整体和系统角度探讨驴胶补血颗粒升高白细胞的作用机制。通过TCMSP数据库和文献挖掘筛选驴胶补血颗粒活性成分,利用DRAR-CPI服务器、GeneCards和CoolGeN数据库预测和筛选驴胶补血颗粒活性成分升白的作用靶点。采用Cytoscape软件构建活性成分-作用靶点网络,使用String数据库和Cytoscape软件绘制蛋白相互作用网络,通过SystemsDockWebSite对成分与靶点进行分子对接验证。采用DAVID数据库对靶点分别进行GO和KEGG通路分析,通过DisGeNET数据库对靶点所属的类型进行归属。筛选得到驴胶补血颗粒49个活性成分,涉及89个作用靶点。网络分析结果表明,驴胶补血颗粒主要涉及嘌呤核糖核苷的合成、中性粒细胞凋亡调控及氧化应激等生物过程,通过调节metabolic、cancer、tuberculosis、PI3K-Akt signaling等多条通路发挥升高白细胞作用。本研究结果反映了驴胶补血颗粒多成分-多靶点-多途径的作用机制特点,为驴胶补血颗粒升高白细胞作用机制的深入研究奠定了基础。The mechanism of leukocyte elevation activity of Lvjiao Buxue granules was studied by establishing the active components-targets network and protein interactions network and analyzing the functions and pathways of targets. The main active ingredients of Lvjiao Buxue granules were obtained by TCMSP and literature excavation. Based on the DRAR-CPI, GeneCards and CoolGeN, the active components of Lvjiao Buxue granules were predicted and screened. Cytoscape software was used to construct the drug-active components-target network, and a protein database was constructed by using String database and Cytoscape software. The relation of the main active ingredients and targets were validated by Systems Dock Web Site. The GO and KEGG pathways involved in the targets were analyzed by DAVID databases. Using DisGeNET database to attribute the type of targets. The results showed that 49 active components and 89 targets of Lvjiao Buxue granules were involved. The network results showed that the composition of purine ribonucleosides, the regulation of cell death, especially the biological processes such as neutrophil and oxidative stress were mainly involved in the regulation of metabolic, cancer, tuberculosis, PI3 K-Akt signaling and many other pathways to play its elevating leukocytes effect. This study reflects the characteristics of multi-components-multi-targets and multi-pathways of Lvjiao Buxue granules, which laid the foundation for further research into the mechanism of leukocyte elevation activity of Lvjiao Buxue granules.

关 键 词：驴胶补血颗粒 白细胞减少症 网络药理学 分子对接 蛋白相互作用 

分 类 号：R966[医药卫生—药理学]