茶多糖对糖尿病肾病小鼠肾脏氧化应激损伤的保护作用研究  

Protective effect of tea polysaccharides on the oxidative stress in kidneys of diabetic mice with nephropathy

作　　者：贾亮亮[1,2] 金桂兰[1,2] 彭官良 郑铁骑[1] 邢翔飞[1,2] 奚炜[1,2] JIA Liang-liang;JIN Gui-lan;PENG Guan-liang;ZHENG Tie-qi;XING Xiang-fei;Xi Wei(Department of Pharmacy,The People＇s Hospital of China Three Gorges University,Yichang Hubei 443000;Institute of Pharmaceutic Preparation,China Three Gorges University,Yichang Hubei 443000)

机构地区：[1]三峡大学人民医院药学部,湖北宜昌443000 [2]三峡大学药物制剂研究所,湖北宜昌443000

出　　处：《中南药学》2018年第10期1388-1392,共5页Central South Pharmacy

基　　金：湖北省卫生计生委中医药中西医结合科研指导项目(No.2017-40);宜昌市科技研究与开发项目(No.A12301-21)

摘　　要：目的探讨茶多糖对糖尿病肾病小鼠肾脏的保护作用。方法采用腹腔注射链脲佐菌素建立糖尿病肾病小鼠模型。随机分为空白对照组、模型组、茶多糖低剂量组、茶多糖高剂量组、卡托普利阳性对照组。给药8周后测定各组小鼠血糖,24 h尿蛋白及血清中血肌酐、尿素氮水平;提取肾脏组织测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)的活性以及丙二醛(MDA)的含量,并对肾脏组织中SOD1、SOD2、SOD3、GPX1mRNA的表达进行测定。结果与空白对照组相比,模型组血糖、24 h尿蛋白、血肌酐及尿素氮明显升高。与模型组相比,茶多糖各剂量组24 h尿蛋白、血肌酐及尿素氮明显下降。同时,茶多糖给药组还可明显提高糖尿病肾病小鼠肾脏内SOD、GPX活性,降低MDA含量,上调肾脏组织中SOD1和GPX1 mRNA的表达。结论茶多糖可以明显改善糖尿病肾病小鼠发病过程中的生化指标,这可能与其上调SOD1、GPX1mRNA的表达,提高肾脏组织中SOD和GPX的活性以及降低MDA含量,调节氧化-抗氧化系统的平衡有关。Objective To determine the renoprotective effect of tea polysaccharides（TPS） on diabetic nephropathy（DN） mice. Methods The DN mice model was induced by intraperitoneal injection of streptozotocin（STZ）. The mice were divided into 5 groups randomly： a normal control group, a model group, a low dose TPS group（200 mg·kg-1）, a high dose TPS group（400 mg·kg-1）, and a captopril（3.13 mg·kg-1） treatment group. The mice were killed at the end of 8 th week. Fasting blood glucose（FBG）, serum creatinine（Scr）, blood urea nitrogen（BUN） and 24 h urine protein were examined by biochemistry methods. The content of MDA and activity of SOD and GPX in the renal tissue were determined by test-kit. The mRNA expressions of SOD1, SOD2, SOD3 and GPX1 were detected by Realtime PCR. Results Compared with the normal control group, the values of FBG, Scr, BUN and 24 h urine protein of the model group were sharply increased. Compared with the model group, the values of Scr, BUN and 24 h urine protein in different TPS dosage treatment groups were significantly decreased. TPS different dosage treatments increased the levels of SOD and GPX and decreased the content of MDA. TPS upregulated the expression levels of SOD1 and GPX1 mRNA in the kidney of diabetic mice with nephropathy. Conclusion TPS may significantly ameliorate the biochemical indicators of the model mice by upregulating the expression levels of SOD1 and GPX1 mRNA in the kidney.

关 键 词：茶多糖 糖尿病肾病 氧化损伤 

分 类 号：R285[医药卫生—中药学]