高效液相色谱法测定十一味草果丸中5种成分的含量  

Content determination of 5 components in 11-taste grass fruit pills by HPLC

作　　者：何启香 常立德 王迪科 毛培芳 HE Qi-xiang;CHANG Li-de;WANG Di-ke;MAO Pei-fang(Food and Drug Inspection Institute of Haixi Prefecture,Delingha Qinghai 817099)

机构地区：[1]青海省海西州食品药品检验所,青海德令哈817099

出　　处：《中南药学》2018年第10期1437-1440,共4页Central South Pharmacy

摘　　要：目的建立高效液相色谱(HPLC)法测定十一味草果丸中麻黄碱、伪麻黄碱、没食子酸、木香烃内酯、去氢木香内酯的含量。方法采用SB-C_(18) Analytical色谱柱(250 mm×4.6 mm,5μm),柱温为30℃,用甲醇(A)-乙腈(B)-0.05 mol·L^(-1)磷酸二氢钾溶液(用磷酸调节pH至3.0±0.1)(C)梯度洗脱,流速为1.0 mL·min^(-1);麻黄碱、伪麻黄碱的检测波长为210 nm;没食子酸、木香烃内酯、去氢木香内酯的检测波长为254 nm。结果麻黄碱、伪麻黄碱、没食子酸、木香烃内酯、去氢木香内酯的线性范围分别为4.12～41.2μg·mL^(-1)(r=0.9996)、3.58～35.8μg·mL^(-1)(r=0.9994)、7.11～71.1μg·mL^(-1)(r=0.9993)、8.04～80.4μg·m L^(-1)(r=0.9999)、7.58～75.8μg·mL^(-1)(r=1.000);平均回收率分别为90.6%(RSD=1.2%)、90.5%(RSD=0.72%)、98.0%(RSD=1.1%)、95.7%(RSD=0.96%)、94.0%(RSD=1.0%);精密度、重复性及稳定性试验的结果可靠(RSD <2%)。结论该方法准确、简便,可用于该制剂中麻黄、诃子、木香3味药材含量的测定。Objective To establish an HPLC method to determine ephedrine, pseudoephedrine, gallic acid, costunolide, and dehydrocostuslactone in 11-taste grass fruit pills. Methods SB-C_（18） analytical HPLC column（250 mm×4.6 mm, 5 μm） was used and the column temperature was 30 ℃. Gradient elution was conduced with methanol（A）-acetonitrile（B）-0.05 mol·L^-1 potassium dihydrogen phosphate solution（phosphoric acid was used to adjust pH to 3.0±0.1）（C）, with the flow rate of 1.0 mL·min^-1. The detection wavelength of ephedrine and pseudoephedrine was 210 nm, while that of gallic acid, costuslactone, and dehydrocostuslactone was 254 nm. Results The linearity of ephedrine, pseudoephedrine, gallic acid, costunolide, and dehydrocostuslactone was 4.12-41.2 μg·mL^-1（r = 0.9996）, 3.58-35.8 μg·mL^-1（r = 0.9994）, 7.11-71.1 μg·mL^-1（r = 0.9993）, 8.04-80.4 μg·mL^-1（r = 0.9999）, and 7.58-75.8 μg·mL^-1（r = 1.000）. The average recovery was at 90.6%（RSD = 1.2%）, 90.5%（RSD = 0.72%）, 98.0%（RSD = 1.1%）, 95.7%（RSD = 0.96%）, and 94.0%（RSD= 1.0%）, respectively. Conclusion The method is simple and accurate, and can be used to determine Ephedra equisetina Bge. Terminalia chebula Petz, Radix Aucklandiae of the 11-taste grass fruit pills.

关 键 词：高效液相色谱法 十一味草果丸 麻黄碱 伪麻黄碱 没食子酸 木香烃内酯 去氢木香内酯 

分 类 号：R917[医药卫生—药物分析学]