高速逆流色谱分离雨生红球藻中虾青素的工艺优化  

Optimization and Separation of Astaxanthin from Haematococcus pluvialis by High-Speed Counter-Current Chromatography

作　　者：白曼利 王海琪 伍菱 陈艳红 黄高凌 杜希萍 BAI Manli;WANG Haiqi;WU Ling;CHEN Yanhong;HUANG Gaoling;DU Xiping(College of Food and Biological Engineering,Jimei University,Xiamen 361021,Fujian,China;Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering,Xiamen 361021,Fujian,China;Research Center of Food Biotechnology of Xiamen City,Xiamen 361021,Fujian,China;Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed,XiamenSouthern Ocean Technology Center of China,Xiamen 361021,Fujian,China)

机构地区：[1]集美大学食品与生物工程学院,福建厦门361021 [2]福建省食品微生物与酶工程重点实验室,福建厦门361021 [3]厦门市食品与生物工程技术研究中心,福建厦门361021 [4]厦门南方海洋研究中心海藻资源化利用与深加工重点实验室,福建厦门361021

出　　处：《激光生物学报》2018年第5期460-466,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(31501448);福建省科技厅重点项目(2018N0019)

摘　　要：应用高速逆流色谱(HSCCC)进行了雨生红球藻中虾青素的分离制备工艺优化,结果最优条件为正己烷∶乙酸乙酯∶乙醇∶水(6. 5∶5∶6. 5∶3,v/v/v/v)作为两相溶剂系统,以下相为固定相,上相为流动相,转速850 r/min,流速3 mL/min,温度25℃,上样浓度10 mg/mL,上样量10 mL。进一步应用高效液相色谱、质谱并与标准品比对,对所得虾青素进行鉴定。本文的研究结果为应用HSCCC高效制备雨生红球藻虾青素提供了技术支持。High-speed counter-current chromatography （HSCCC） was developed for separation and purification ofastaxanthin from Haematococcus pluvialis. The optimized two-phase solvent system, revolution speed, flow rate,temperature, sample concentration and sample volume were n-hexane-ethyl acetate-ethanol-water（6. 5∶ 5∶ 6. 5∶ 3, v / v /v / v） , 850 r / min, 3 mL / min, 25 ℃, 10 mg / mL and 10 mL, respectively. The lower phase was used as stationaryphase, while the upper phase was the mobile phase of HSCCC. Furthermore, the compound collected from HSCCCanalyzed by high performance liquid chromatography（HPLC） , mass spectrometry（MS） and standard and was confirmedas astaxanthin. This paper can provide technical support to efficient preparation of astaxanthin from H. pluvialis usingHSCCC.

关 键 词：高速逆流色谱(HSCCC) 虾青素 雨生红球藻 分离 制备 

分 类 号：R917[医药卫生—药物分析学]