黏蛋白1和上皮细胞黏附分子mRNA在肺癌患者胸腔积液中的表达水平及临床意义  

Expression level and clinical significance of MUC1 and EpCAM mRNA in pleural effusion of patients with lung cancer

作　　者：陈捷[1] 黄伟萍[1] 翁丹丹[2] CHEN Jie;HUANG Wei-ping;WENG Dan-dan(Clinical Laboratory,the People＇s Hospital of Yuyao,Yuyao,Zhejiang 315400,China)

机构地区：[1]余姚市人民医院检验科,浙江余姚315400 [2]余姚市人民医院内六病区,浙江余姚315400

出　　处：《中国卫生检验杂志》2018年第20期2496-2498,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨MUC1和EpCAM mRNA在肺癌患者胸腔积液中的表达水平及其临床意义。方法选取产生胸腔积液的肺癌患者92例作为研究对象,根据脱落细胞学或胸腔镜活检结果分为良性胸腔积液组和恶性胸腔积液组。采用qRT-PCR测定MUC1和EpCAM mRNA表达水平。结果肺癌患者恶性胸腔积液组患者的MUC1和EpCAM mRNA表达水平显著高于良性胸腔积液组,差异均有统计学意义(P <0. 01)。ROC分析显示,胸腔积液MUC1 mRNA的曲线下面积(AUC)为0. 79,灵敏度为76. 2%,特异度为85. 7%;胸腔积液EpCAM mRNA的AUC为0. 85,灵敏度为81. 5%,特异度为78. 4%。结论胸腔积液MUC1和EpCAM mRNA表达水平的检测对良性与恶性胸腔积液的鉴别诊断有一定的辅助性参考价值。Objective To investigate the expression level and clinical significance of MUC1 and EpCAM mRNA in pleural effusion of patients with lung cancer. Methods 92 cases of lung cancer with pleural effusion were selected as the research objects, and according to exfoliative cytology or thoracoscopic biopsy results, they were divided into benign pleural effusion group and malignant pleural effusion group. The levels of MUC1 and EpCAM mRNA were measured by qRT-PCR. Results The levels of MUC1 and EpCAM mRNA in patients with malignant pleural effusion of lung cancer were significantly higher than those of benign pleural effusion, and the difference was statistically significant （ P 〈 0.01 ） ： ROC analysis showed that the area under the curve（AUC） of MUC1 mRNA in pleural effusion was 0.79, the sensitivity was 76.2% and the specificity was 85.7%. The AUC of pleural effusion EpCAM mRNA was 0.85, the sensitivity was 81.5% and the specificity was 78.4%. Conclusion The detection of MUC1 and EpCAM mRNA levels in pleural effusion has a certain reference value for the diagnosis of benign and malignant pleural effusions.

关 键 词：黏蛋白1 上皮细胞黏附分子 肺癌 胸腔积液 

分 类 号：R736.3[医药卫生—肿瘤]