长链非编码RNA H19,EZH2,Ki-67及突变型P53联合检测在子宫颈癌诊断中的意义  

Expression and significance of long non-coding RNA H19, EZH2, Ki-67, and mutant P53 in cervical cancer

作　　者：辜卫红[1] 余敏敏[2] 袁智民[1] 吴玲[1] 邱峰龙[1] 项媛媛[1] 方芳[1] 杨慧[1] GU Weihong;YU Minmin;YUAN Zhimin;WU Ling;QIU Fenglong;XIANG Yuanyuan;FANG Fang;YANG Hui(Department of Obstetrics and Gynecology,Huaian Maternal and Child Health Care Hospital,Huaian Jiangsu 223002;Department of Obstetrics and Gynecology,The Second Affiliated Hospital,Nanjing 210003,China)

机构地区：[1]淮安市妇幼保健院妇产科,江苏淮安223002 [2]南京市第二医院妇产科,南京210003

出　　处：《临床与病理杂志》2018年第9期1823-1827,共5页Journal of Clinical and Pathological Research

基　　金：国家自然科学基金(81472431);淮安市科技计划项目(HAS2015024)~~

摘　　要：目的:研究长链非编码RNA(long non-coding RNA,lncRNA) H19,EZH2,Ki-67,突变型P53联合检测在子宫颈癌诊断中的意义。方法:取子宫颈活检或手术组织标本进行病检,其中50例子宫颈癌为研究组,50例非瘤子宫颈为对照组。采用qRT-PCR检测lncRNA H19在各组组织中的表达量。利用免疫组织化学SP法检测以上2组宫颈组织石蜡标本中EZH2,Ki-67,突变型P53的表达。应用SPSS 13.0软件包统计分析各组H19,EZH2,Ki-67及突变型P53的表达与子宫颈癌临床和病理诊断结果的相关性。结果:研究组H19的表达量低于对照组(P<0.05);研究组EZH2,Ki-67,突变型P53阳性表达量高于对照组,差异具有统计学意义(P<0.05)。结论:临床工作中联合检测H19,EZH2,Ki-67,突变型P53更有利于子宫颈癌的诊断。Objective： To assess the expression and clinical significance of long non-coding RNA（lncRNA） H19, EZH2, Ki-67, and mutant P53 in cervical cancer. Methods： Cervical biopsy or surgical tissue specimens were divided into 2 groups according to pathological diagnosis： 50 cases of cervical cancer were regarded as an experiment group and 50 cases of non-cancerous cervical were a control group. qRT-PCR was used to evaluate the expression levels of lncRNA H19. Immunohistochemistry（SP method） was used to evaluate the expression levels of EZH2, Ki-67 and mutant P53 in fixed specimens. Data were analyzed by the SPSS 13.0 software. The associations of lncRNA H19, EZH2, Ki-67 and mutant P53 levels with clinicopathological features of cervical cancer were assessed. Results： LncRNA H19 were significantly low expressed in cervical cancer tissues compared with those of the control groups（P〈0.05）. EZH2, mutant P53 and Ki-67 amounts expressed positively in cervical cancer tissues. Conclusion： It is beneficial to combine the detection of lncRNA H19, EZH2, Ki-67 and mutant P53 for the diagnosis of cervical cancer.

关 键 词：子宫颈癌 长链非编码RNA H19 EZH2 核增殖抗原Ki-67 突变型P53 

分 类 号：R737.33[医药卫生—肿瘤]