HPLC法测定蒙药嘎日迪-5丸中没食子酸的含量  

To determinate the contents of Total gallic acid in Mongolian Medicine garidi-5 Pill by HPLC

作　　者：安明[1] 赵国君[2] 方鑫 邬国栋[1] An Ming;Zhao Guo Jun;Fang Xin;Wu Guo Dong(Baotou Medical College,Baotou 014060,China)

机构地区：[1]包头医学院,内蒙古包头014060 [2]包头市第四医院,内蒙古包头014030

出　　处：《中国民族医药杂志》2018年第9期41-43,共3页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：内蒙古自治区高等学校科学研究项目(NJZY18197);包头医学院"花蕾计划"研究项目

摘　　要：目的:建立高效液相色谱法测定嘎日迪-5丸中没食子酸含量的方法。方法:采用采用高效液相色谱法对没食子酸进行含量测定,其色谱条件为:C18色谱柱(250mm×4. 6mm,5μm),柱温30℃,流动相为乙腈-0. 1%三乙胺的0. 1%磷酸溶液(1:99),检测波长273nm,流速1. 0 mL/min。结果:色谱峰分离情况良好,没食子酸在0. 1826~1. 0956μg/mL范围内与峰面积呈良好的线性关系(r=0. 999),平均加样回收率为(n=9)为102. 88%(RSD=1. 87%)。结论:本法所建立的含量测定方法简便准确、专属性强、重复性好,可用于嘎日迪-5丸的质量控制。Objective To determinate the contents of gallic acid in Garidi-5 pills. Methods An HPLC method was used and Pheomenex Luna C18 was selected（ 250 × 4. 6 mm × 5μm）,acolumn temperature was 30℃,the mobile phase was consisted of acetonitrile-0. 1% phosphoric acid solution of 0. 1% triethylamine（ 1： 99） were mobile phase the detecting wave length and velocity were 273 nm and 1. 0 mL/min repectively. Results There were established a good linear relation between 0. 1826 ～ 1. 0956μg/mL of gallic acid,r = 0. 99999,the average recovery was 102. 88%,RSD was1. 87%. Conclusion The method had the advantages of simple and easy operation,sensitive and accurate and exhibited a good separation effect. The methods were can be used to determination of contents and quality control of the Garidi-5 Pill.

关 键 词：蒙药 嘎日迪-5丸 高效液相色谱法 没食子酸 

分 类 号：R291.2[医药卫生—民族医学]