用SE-iFISH技术检测肝细胞癌患者外周血循环中的肿瘤细胞  

SE-iFISH used in the detection of circulating tumor cells in patients of hepatocellular carcinoma

作　　者：石军[1] 叶光强 柯雨尼 叶春辉 杨世伟[1] 童翾 徐静[2] 董家鸿[1] Shi Jun;Ye Guangqiang;Ke Yuni;Ye Chunhui;Yang Shiwei;Tong Xuan;Xu Jing;Dong Jiahong(HPB Center,Beijing Tsinghua Changgung Hospital,Tsinghua University,Beijing 102218,China)

机构地区：[1]清华大学附属北京清华长庚医院肝胆胰中心,102218 [2]广西医科大学第一附属医院肝胆外科

出　　处：《中华普通外科杂志》2018年第10期865-868,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（81360372）;北京市医院管理局l临床医学发展专项基金资助项目（ZYLX201712）

摘　　要：目的探讨用SE-iFISH检测肝细胞癌患者手术前后外周血中循环肿瘤细胞（circulating tumor cells，CTCs）数量及亚型与患者临床特征的相关性。方法收集2015年6月至2016年6月行根治性切除的20例肝细胞癌患者的临床资料及手术前后的外周血，采用SE-iFISH分离和鉴定CTCs，检测CTCs数量和CTCs亚型，分析CTCs数量与患者的生存关系。结果20例肝细胞癌患者中共检测出CTCs 347个，其中3倍体114个，4倍体64个，≥5倍体165个。术前CTCs数量以及术前3倍体数量和脉管癌栓（Z1=-2．080，P=0．037，Z3=-2．321，P=0．020）以及TNM分期（Z2=-2．148，P=0．032，Z4=-2．526，P=0．012）都具有显著相关性。CTCs高表达组的无瘤生存期明显短于CTCs低表达组（X^2 1=7．486，P=0．006,X^2=12．056，P=0．001）。结论通过SE-iFISH检测术后CTCs数量及其亚型有助于预测肝细胞癌疗效以及预后。Objective To investigate the correlation between patients clinical characteristics and the number and subtype of circulating tumor cells （CTCs） from peripheral blood of perioperative hepatocellular carcinoma （HCC）patients by SE-iFISH. Methods 20 HCC patients undergoing radical resection were enrolled from June 2015 to June 2016. The SE-iFISH technique was used to separate and identify circulating tumor ceils. The pathology and clinical data were used to evaluate patients survival in combination with CTCs characteristics. Results A total of 347 CTCs were detected, of which 114 were triploid, 64 were tetraploid, and 165 were pentaploid. The number of preoperative CTCs and the number of preoperative triploids was significantly correlated with the presence of vascular tumor emboli （Z1=-2. 080 ,P = 0. 037, Z3= -2.321,P=0.020）and TNM staging（Z2 = -2.148,P=0.032,Z4 = -2.526,P=0.012）. Postoperative patients disease-free survival in high CTCs detection group was significantly shorter than that of CTCs low expression group （X^21=7.486, P=0.006 ,X^22=12. 056, P=0.001 ）. Conclusion Detection of the number and the specific subtypes of CTCs with SE-iFISH strategy in patients with HCC help predict treatment efficacy and prognosis.

关 键 词：癌 肝细胞 肿瘤细胞 循环 存活率分析 

分 类 号：R735.7[医药卫生—肿瘤]