麻醉对肥胖复合牙周炎模型副作用的研究  

Anesthetic side effects on the diet-induced obese mice treated for ligation-induced periodontitis

作　　者：余挺[1] 赵莉[2] 章锦才 轩东英 YU Ting ; ZHAO Li; ZHANG Jincai; XUAN Dongying(Department of Periodontology , Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China)

机构地区：[1]广州医科大学附属口腔医院牙周科广州口腔疾病研究所口腔医学重点实验室,广东广州510140 [2]中山大学光华口腔医学院附属口腔医院口腔修复科广东省口腔医学重点实验室,广东广州510055 [3]中国科学院大学存济医学院,北京100049 [4]杭州口腔医院牙周科,浙江杭州310006

出　　处：《口腔医学》2018年第10期885-889,共5页Stomatology

基　　金：国家自然科学基金(81700985;81371151;81470750);广州市卫生和计划生育科技项目(20181A011102)

摘　　要：目的探索麻醉副作用相关内脏病变在饮食诱导型肥胖(DIO)联合结扎诱导牙周炎(LIP)模型中的分布情况。方法建立DIO与LIP(牙周结扎5或10d)联合小鼠模型(n=120),LIP于4%水合氯醛腹腔麻醉下完成,大体解剖评估腹腔内脏病变。结果麻醉副作用相关内脏病变累及14.8%(17/115)小鼠,涉及附睾、内脏脂肪、肾、脾、肝,且发生于同一侧。DIO小鼠的内脏病变、粘连的占比分别高于体重正常组1倍(18.1%vs. 9.3%,P=0.31)和2倍(8.3%vs. 2.3%,P=0.37),但此差异无统计学意义。10 d组的内脏病变、粘连的占比分别高于5 d组1倍(19.0%vs. 10.5%,P=0.31)和4倍(10.3%vs. 1.8%,P=0.12),但此差异无统计学意义。结论建立肥胖与牙周炎的联合模型时,应慎重评估AAEs对两者相关性的混杂影响。Objective To explore the distribution of anesthetic adverse effects （AAEs） related visceral organ lesions in combined diet-induced obese （DIO） and ligation-induced periodontitis （LIP） models. Methods Combined DIO（16-week on diet） and LIP（5-or 10-day ligation） mouse models were established （n = 120）. Abdominal organ lesions related to AAEs were assessed by general necropsy. Results AAEs-related organ lesions were found in 14.8%（17/115） of the mice. The involved organs included epididymis,visceral adipose tissue,kidney,spleen and liver,which were all lesioned on the same side. Despite of no significance,the proportions of organ lesions and adhesion were respectively 1（18.1% vs. 9.3%,P = 0.31） and 2（8.3% vs. 2.3%,P = 0.37） times higher,respectively,in DIO mice than those in normal-weight controls and were respectively 1 （19.0% vs. 10.5%,P = 0.31） and 4 （10. 3% vs.1.8%,P = 0.12） times higher,respectively,after 10 days of ligation than those after 5 days of ligation. Conclusions When establishing combined obesity and periodbntitis models,potential confounding effects of AAEs on their correlation should be critically assessed.

关 键 词：麻醉 动物模型 肥胖 牙周炎 副作用 

分 类 号：R781.4[医药卫生—口腔医学]