胃癌中miR-203的表达水平及其与临床病理参数和预后的关系  

Expression level of miR-203 in gastric cancer and its associations with clinicopathological parameters and prognosis

作　　者：季超[1] 王梦桥[1] 黄河[1] JI Chao;WANG Meng-qiao;HUANG He(Department of Gastrointestinal Surgery,Fifth People＇s Hospital of Chengdu,Chengdu,Sichuan 611130,China)

机构地区：[1]成都市第五人民医院胃肠外科,四川成都611130

出　　处：《中国临床研究》2018年第7期870-874,共5页Chinese Journal of Clinical Research

基　　金：四川省卫生厅科研基金项目(140094)

摘　　要：目的检测微小核糖核酸(microRNA,miR)-203在胃癌组织中的表达水平,探讨其与胃癌患者临床病理参数和预后的关系。方法采用实时荧光定量PCR(qRT-PCR)技术检测86例胃癌组织及相应癌旁组织中miR-203表达水平,分析miR-203表达水平对胃癌患者临床病理参数和3年总生存率的影响。结果 miR-203在胃癌组织中相对表达水平明显低于癌旁组织,差异有统计学意义(0.34±0.06 vs 1.02±0.19,P<0.05)。miR-203的表达与胃癌患者肿瘤大小、病理分化程度及TNM分期有关(P均<0.01),与患者年龄、性别及淋巴结转移无关(P均>0.05)。Kaplan-Meier生存分析表明,miR-203低表达者3年生存率显著低于miR-203高表达者3年生存率(46.38%vs 88.24%,P<0.01)。COX分析结果表明,miR-203低表达、TNM分期高是影响胃癌患者预后的独立危险因素(HR=0.477,95%CI:0.298~0.762,P<0.01;HR=2.503,95%CI:1.110~5.644,P<0.01)。结论 miR-203在胃癌组织中低表达,与患者肿瘤大小、病理分化程度、TNM分期及3年生存率有关,是影响胃癌患者预后的独立危险因素。Objective To detect the expression level of microRNA（ miR）-203 in gastric cancer tissue and explore its association with the clinicopathological parameters and prognosis of gastric cancer patients. Methods Real-time fluorescence quantitative polymerase chain reaction（ qRT-PCR） was used to detect miR-203 expression in gastric cancer tissues and corresponding paracancerous tissues of 86 patients. The influences of miR-203 expression level on the clinicopathological parameters and the 3-year total survival rate of gastric cancer patients were analyzed. Results The relative expression level of miR-203 in gastric cancer tissues was significantly lower than that of paracancerous tissues（ 0. 34 ± 0. 06 vs 1. 02 ± 0. 19,P〈0. 05）. The expression of miR-203 was associated with the size of tumor,the degree of pathological differentiation and TNM staging（ all P〈0. 01） and not associated with the age,sex and lymph node metastasis（ all P〈0. 05）. Kaplan-Meier survival analysis showed that the 3-year survival rate in patients with low expression of miR-203 was significantly lower than that in patients with high expression of miR-203（ 46. 38% vs 88. 24%,P〈0. 01）. COX analysis showed that miR-203 low expression（ HR = 0. 477,95% CI： 0. 298-0. 762,P〈0. 01） and high TNM stage（ HR =2. 503,95% CI： 1. 110-5. 644,P〈0. 01） were the independent risk factors affecting the prognosis of gastric cancer patients. Conclusions The expression level of miR-203 in gastric cancer tissues is low,which is related to the tumor size,pathological differentiation,TNM staging and 3-year survival rate,and it is an independent risk factor influencing prognosis of gastric cancer patients.

关 键 词：胃癌 微小核糖核酸-203 临床病理参数 3年生存率 预后 

分 类 号：R735.25[医药卫生—肿瘤]