高效液相色谱-柱后衍生法测定食品中黄曲霉毒素B1、B2、G1、G2  

Determination of aflatoxin B1, B2, G1, G2 in food by high performance liquid chromatography with Post-column derivatization

作　　者：王志强[1] WANG Zhiqiang(Anyang Center for Disease Control and Prevention,Henan 455000,China)

机构地区：[1]安阳市疾病预防控制中心,河南安阳455000

出　　处：《河南预防医学杂志》2018年第11期828-831,共4页Henan Journal of Preventive Medicine

摘　　要：目的建立适用于基层实验室适用的高效液相色谱-柱后衍生法测定食品中黄曲霉毒素B1、B2、G1、G2的检测方法。方法试样经乙腈—水提取,离心,取上清液过多功能净化柱,净化液用氮吹吹干,20%乙腈—水溶液定容,过0.22μm滤膜后进样,柱后碘溶液(0.05%)衍生,荧光检测器检测,外标法定量。结果在0.01 ng/mL～40 ng/mL时,线性关系良好,相关系数在0.999以上,加标回收率在85%～95%之间,相对标准偏差3.87%～5.00%。结论该方法能够准确可靠的测食品中黄曲霉毒素B1、B2、G1、G2的含量,且灵敏度较高,0.05%碘溶液柱后衍生方法对仪器条件要求不高,方法操作简单,便于满足基层实验室对黄曲霉毒素B1、B2、G1、G2的测定。Objective To establish a method for the determination of aflatoxin B1, B2, G1 and G2 in food by high performance liquid chromatography（HPLC）. Methods The sample extracted by acetonitrile-water, centrifugation,and took the supernatant fluid pass multifunctional purification column.Purification of liquid nitrogen blow dry, 20 %acetonitrile water solution volume, 0.22 μm filter before injection.After column iodine solution（0.05 %） derivatives,Detection of fluorescence detector, external standard method for quantitative. Results In the 0.01 ng/mL-40 ng/mL,the linear relationship was good, the correlation coefficient was above 0.999, and the recovery rate was 85 %-95 %,the relative standard deviation 3.87 % —5.00 %.Conclusion This method can be used to determine the content of aflatoxin B1, B2, G1 and G2 in the beer, peanuts, corn and its products,and the sensitivity is higher.After column iodine solution（0.05%） derivatives method is simple and easy to operate. It is convenient for the determination of aflatoxin B1, B2, G1 and G2 in the primary laboratory.

关 键 词：高效液相色谱 黄曲霉毒素B1、B2、G1、G2 柱后衍生 

分 类 号：R155.5[医药卫生—营养与食品卫生学]