北京油鸡羽速自别雌雄配套系构建及应用研究  

Establishment and Application of Feathering-autosexing Lines in Beijing You Chicken

作　　者：初芹[1,2] 张剑 张尧[1,2] 晏志勋 曾另超[1,2] 王海宏 耿爱莲[1,2] 刘华贵 CHU Qin;ZHANG Jian;ZHANG Yao;YAN Zhixun;ZENG Lingchao;WANG Haihong;GENG Ailian;LIU Huagui(Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;Daxing Experiment Station of National Broiler Industry Technology System,Beijing 102602)

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京100097 [2]国家肉鸡产业技术体系大兴综合试验站,北京102602

出　　处：《中国家禽》2018年第19期12-16,共5页China Poultry

基　　金：北京市科技计划项目(D171100007817005;D171100007817003);国家自然科学基金青年科学基金项目(3140130107);现代农业产业技术体系建设专项资金(CARS-41-Z01);北京市农林科学院科技创新能力建设专项(KJCX20170105)

摘　　要：研究建立了一种荧光定量PCR方法对公鸡慢羽基因型进行检测和判定,进而快速实现了北京油鸡慢羽纯系的构建。并进一步开展了两种四系杂交配套试验,对羽速自别雌雄的准确性进行验证。结果显示,北京油鸡两种配套系自别雌雄鉴别率为公雏99%以上,母雏97.98%以上,该方法可以在生产中应用。In the present study, a fluorescent quantitative PCR method was established for genotyping late-feathering K locus in male birds. Using this molecular detecting platform, a late-feathering pure strain of Beijing You chicken was established in which all birds were homozygous at the late-feathering loci. Furthermore, two crossbred combinations were used to test the autosexing accuracy. The results showed that the sexing accuracy for male and female chicks were above 99% and 97.98% respectively, and the autosexing technology could be used in commercial Beijing You chicken.

关 键 词：北京油鸡 羽速 雌雄自别 慢羽基因 

分 类 号：S831.2[农业科学—畜牧学]