桃小食心虫和梨小食心虫幼虫肠道细菌多样性  

Gut bacterial community diversity in Carposina sasakii and Grapholitha molesta

作　　者：李艳艳[1] 孙丽娜[1] 田志强 韩海斌[2] 张怀江[1] 仇贵生[1] 闫文涛[1] 岳强[1] LI Yan-yan;SUN Li-na;TIAN Zhi-qiang;HAN Hai-bin;ZHANG Huai-jiang;QIU Gui-sheng;YAN Wen-tao;YUE Qiang(a Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100,Liaoning,China;Institute of Grassland Research,Chinese Academy of Agricul-tural Sciences,Hohhot 010010,China)

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100 [2]中国农业科学院草原研究所,呼和浩特010010

出　　处：《应用生态学报》2018年第10期3449-3456,共8页Chinese Journal of Applied Ecology

基　　金：国家重点研发计划项目(2017YFD0201000);中央级公益性科研院所基本科研业务费专项(1610182016004)资助~~

摘　　要：为明确桃小食心虫和梨小食心虫幼虫肠道细菌的结构和多样性,本研究利用Illumina HiSeq技术对饲喂金冠苹果的桃小食心虫和梨小食心虫幼虫肠道细菌的16S rRNA V4变异区进行测序与分析.结果表明:共获得桃小食心虫幼虫肠道细菌229043条reads,聚类为2112个OTUs,注释得到27个门,65个纲,124个目,205个科,281个属;共获得梨小食心虫幼虫肠道细菌240389条reads,聚类为957个OTUs,注释得到22个门,46个纲,89个目,145个科,180个属.桃小食心虫幼虫肠道细菌以变形菌门(87.98%±5.29%)、厚壁菌门(3.91%±1.19%)和放线菌门(1. 04%±0. 47%)为主;梨小食心虫肠道细菌以变形菌门(50. 06%±19.56%)、厚壁菌门(32.02%±8.48%)和蓝细菌门(25.24%±10.28%)为主.两种食心虫在门、纲、目、科、属、种水平上均存在显著差异.从本研究结果看,即使均以苹果为寄主,两种食心虫幼虫肠道细菌结构和多样性也存在显著差异,桃小食心虫幼虫肠道细菌多样性比梨小食心虫丰富,这可能与两者不同的取食特性和消化机制有关.本研究结果为后期揭示肠道微生物与两种食心虫相互作用关系奠定了基础.To clarify the community structure and diversity of larval gut bacteria in Carposina sasakii and Grapholitha molesta,the V4 regions of the 16S rDNA genes of intestinal bacteria of C.sasakii and G. molesta larvae which fed by golden delicious apple,were amplified and sequenced by Illumina HiSeq technique. The results showed that a total of 229043 high quality reads of gut bacteria in C. sasakii larvae were obtained and clustered to 2112 OTUs,which were annotated into 27 phyla,65 classes,124 orders,205 families and 281 genera. 240389 reads of G. molesta were produced and clustered to 957 OTUs,which were annotated into 22 Phyla,46 Classes,89 Orders,145 Families and 180 Genera. C. sasakii was dominated by the Proteobacteria（ 87.98% ±5.29%）,Firmicutes（ 3.91% ±1.19%）,Actinobacteria（ 1.04% ±0.47%）,and G. molesta was mainly dominated by Proteobacteria（ 50. 06% ± 19. 56%）,Firmicutes（ 32. 02% ± 8. 48%） and Cyanobacteria（ 25.24%±10.28%）. All of Phylum,Class,Order,Family,Genus,Species of the bacteria were significantly different between C. sasakii and G. molesta. Those results suggested that the gut bacteria community in these two fruit-boring pests was notably different,although they were both fed by apple fruit. The bacteria communities were more complex in C. sasakii than in G. molesta,which might account for different feeding and digestion mechanisms. The results could lay a foundation to reveal the association of the intestinal bacteria with these two fruit borers.

关 键 词：桃小食心虫 梨小食心虫 肠道细菌组成 16S RRNA 高通量测序 

分 类 号：S433[农业科学—农业昆虫与害虫防治]