IL-17A通过NF-κB活化促进HepG2细胞炎症因子分泌  

IL-17A promoting the secretion of inflammatory cytokines in HepG2 cells by activating NF-κB

作　　者：尹文君[1] 王秋平 李绵利 姜孝新[1] Yin Wenjun;Wang Qiuping;Li Mianli(Department of Clinical Laboratory,The First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学第一附属医院检验科 [2]船山实验中学,湖南衡阳421001

出　　处：《湘南学院学报（医学版）》2018年第3期1-4,共4页Journal of Xiangnan University（Medical Sciences）

基　　金：湖南省自然科学基金资助项目(2018JJ3466)

摘　　要：目的探讨白介素-17A(IL-17A)对HepG2细胞炎症因子分泌的影响及分子机制。方法培养Hep G2细胞,分为对照组、重组IL-17A(rIL-17A)组和吡咯烷二硫氨基甲酸酯(PDTC)+rIL-17A组,分别予PBS、rIL-17A、PDTC+rIL-17A处理,荧光实时定量PCR检测白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α) m RNA的表达,酶联免疫吸附法分析上清液中IL-6、TNF-α的水平,Western blot测定细胞核内核因子-κB(NF-κB) p65的表达。结果与对照组比较,rIL-17A组IL-6、TNF-αmRNA表达上调(P<0.01),上清液中IL-6、TNF-α水平增加(P<0.01)。rIL-17A组核内NF-κB p65表达也较对照组增加(P<0.01)。PDTC预处理则抑制rIL-17A作用Hep G2细胞合成分泌IL-6、TNF-α(P<0.05)。结论 IL-17A可促进Hep G2细胞IL-6、TNF-α产生和分泌,其机制可能与激活NF-κB信号通路有关。Objective To investigate the effects of interleukin-17 A（ IL-17 A） on the secretion of inflammatory cytokines in HepG2 cells and the molecular mechanism. Methods HepG2 cells were cultured and randomly divided into control group,recombinant IL-17 A（ rIL-17 A） group,and pyrrolidine dithiocarbamate（ PDTC） ＋rIL-17 A group. Cells in these three groups were treated with PBS,rIL-17 A,and PDTC＋rIL-17 A,respectively. The m RNA expression of interleukin-6（ IL-6） and tumor necrosis factor-α（ TNF-α）was detected by quantitative real-time PCR. The levels of IL-6 and TNF-α in the supernatant were analyzed by enzyme linked immunosorbent assay. Meanwhile,Western blot was used to determine the p65 expression of nuclear zkernel factor-κB（ NF-κB）.Results Compared with control group,the m RNA expression of IL-6 and TNF-α was significantly elevated in rIL-17 A group with an increase in IL-6 and TNF-αlevels in the supernatant（ P〈0.01）. Nuclear NF-κB p65 expression in rIL-17 A group was higher than that in control group（ P〈0.01）. Pretreatment with PDTC inhibited the effects of rIL-17 A on synthesis and secretion of both cytokines in HepG2 cells（ P〈0.05）. Conclusion IL-17 A can promote the production and secretion of IL-6 and TNF-α in HepG2 cells,which may be associated with activation of the NF-κB signaling pathway.

关 键 词：白介素-17A 核因子-ΚB 肝癌 白介素-6 肿瘤坏死因子-α 

分 类 号：R735.7[医药卫生—肿瘤]