CBX3表达在结直肠癌中的临床意义及其机制探索  

Clinical Significance of Expression of CBX3 in Colorectal Cancer and its Possible Mechanism

作　　者：徐浒东 严婷婷[1] 艾罗燕[1] 周超[2] 王争[2] 汤佳音[2] XU Hudong;YAN Tingting;AI Luoyan;ZHOU Chao;WANG Zheng;TANG Jiayin(Division of Gastroenterology and Hepatology,Renji Hospital,School of Medicine,Shanghai Jiao Tong University;Shanghai Institute of Digestive Disease,Shanghai,200001;2 Department of Gastrointestinal Surgery,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shangha)

机构地区：[1]上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所,200001 [2]上海交通大学医学院附属仁济医院胃肠外科

出　　处：《胃肠病学》2018年第10期585-590,共6页Chinese Journal of Gastroenterology

基　　金：上海市卫生和计划生育委员会科研课题面上项目(201540240)

摘　　要：背景:CBX3属于异染色质蛋白家族成员之一,近年研究发现CBX3与肺癌、骨肉瘤、胃癌等多种人类癌症密切相关。目的:探讨CBX3在结直肠癌中的表达及其临床意义,并探索可能的作用机制。方法:选取2011年6月—2012年6月上海交通大学医学院附属仁济医院30例结直肠癌组织及其相应癌旁正常组织。以实时定量PCR和免疫组化法分别检测CBX3 mRNA和蛋白表达。将CBX3过表达质粒和CBX3 siRNA转染人结直肠癌细胞株RKO,以CCK-8法检测细胞增殖,蛋白质印迹法检测CBX3、p53蛋白表达。结果:结直肠癌组织中CBX3 mRNA和蛋白表达均显著高于相应癌旁正常组织,且CBX3蛋白表达与肿瘤大小(P=0. 025)、淋巴结转移(P=0. 013)和TNM分期(P=0. 020)相关。过表达CBX3可有效上调RKO细胞中CBX3 mRNA和蛋白表达,促进细胞增殖,同时上调p53蛋白表达;敲低CBX3可有效下调CBX3 mRNA和蛋白表达,抑制细胞增殖,同时下调p53蛋白表达。结论:CBX3可能通过影响p53表达来促进结直肠癌细胞增殖,进而影响结直肠癌疾病进程,提示CBX3有望成为诊断和治疗结直肠癌的新靶点。Background： CBX3 is a member of heterochromatin protein family. Recent studies indicated that CBX3 was closely related to lung cancer, osteosarcoma, gastric cancer. Aims： To investigate the expression and clinical significance of CBX3 in colorectal cancer, and explore the potential mechanism. Methods： Thirty colorectal cancer patients from June 2011 to June 2012 at Shanghai Renji Hospital were enrolled. Real-time quantitative PCR and immunohistochemistry were used to determine mRNA and protein expressions of CBX3 in colorectal cancer tissues and corresponding paracancerous tissues, respectively. Human colorectal cancer cell line RKO was transfected with overexpressed plasmid or siRNA of CBX3. Cell proliferation was measured by CCK-8 assay, Western blotting was implemented to determine the protein expressions of CBX3 and p53. Results： The mRNA and protein expressions of CBX3 were significantly increased in colorectal cancer tissues than in corresponding paracancerous tissues. Increased protein expression of CBX3 was closely correlated with tumor size （ P =0.025）, lymph node metastasis （ P =0.013） and TNM staging （ P =0.020）. After intervention with overexpressed plasmid of CBX3, the mRNA and protein expressions of CBX3 were efficiently upregulated in RKO cells, cell proliferation and protein expression of p53 were significantly increased. Meanwhile, the mRNA and protein expressions of CBX3 were efficiently downregulated in RKO cells after knockdown of CBX3, resulting in significantly decreased cell proliferation and protein expression of p53. Conclusions： CBX3 may promote the proliferation of colorectal cancer cells via influencing the expression of p53, thus promoting the progression of colorectal cancer. This indicates that CBX3 has the potential to be a new target for diagnosis and therapy of colorectal cancer.

关 键 词：CBX3 结直肠肿瘤 细胞增殖 P53 

分 类 号：R735.34[医药卫生—肿瘤]