自身免疫性肝病患者血清IL-17与IFN-γ、TNF-α及肝功能的相关性研究  

Correlation between Serum IL-17 and IFN-γ,TNF-α and Liver Function in Patients with Autoimmune Liver Disease

作　　者：张爱芸[1] 马娟[1] 张旭[1] 王煜[1] 杨蕾 张艳红[2] ZHANG Aiyun;MA Juan;ZHANG Xu;WANG Yu;YANG Lei;ZHANG Yanhong(Department of Infectious Diseases,the General Hospital of Ningxia Medical University,Yinchuan 750004;Ningxia Medical University,Yinchuan 750004)

机构地区：[1]宁夏医科大学总医院感染疾病科,银川750004 [2]宁夏医科大学,银川750004

出　　处：《宁夏医科大学学报》2018年第7期798-802,共5页Journal of Ningxia Medical University

摘　　要：目的研究自身免疫性肝病(AILD)患者血清白细胞介素-17(IL-17)与干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)及肝功能的关系。方法纳入AILD患者70例,其中自身免疫性肝炎(AIH)患者40例,原发性胆汁淤积性肝硬化(PBC)患者30例,AILD患者为治疗组给予液体静滴加强保肝、退黄及降酶治疗。并选择同期健康体检者20例为对照组。采用酶联免疫吸附法检测治疗前后血清IL-17、IFN-γ、TNF-α含量,并检测血清免疫球蛋白G(IgG)及肝功能相关指标:血清总胆红素(TBIL)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、谷氨酰转移酶(γ-GT)等。结果 1)AIH组、PBC组患者治疗前血清IL-17、TBIL、AST、ALT、γ-GT、IgG水平均高于对照组(P均<0.05)。AIH组、PBC组治疗后患者血清IL-17、TBIL、AST、ALT、IgG水平均低于治疗前(P<0.05),两组γ-GT水平治疗前后差异无统计学意义(P>0.05)。AIH组、PBC组治疗后血清TBIL、AST、ALT、γ-GT水平均高于对照组(P均<0.05),IL-17、IgG水平差异无统计学意义(P>0.05)。2)AIH组、PBC组患者治疗前血清IFN-γ、TNF-α水平均高于对照组(P均<0.05),治疗后与对照组比较差异均无统计学意义(P均>0.05);AIH组、PBC组患者治疗后血清IFN-γ、TNF-α水平均低于治疗前(P均<0.05)。3)70例AILD患者血清IL-17与TBIL、AST、ALT、IgG、γ-GT、IFN-γ、TNF-α均呈正相关关系(P均<0.05)。结论 IL-17在AILD患者血清中水平增高、且与肝功能及IFN-γ、TNF-α关系密切,推断IL-17可能在AIH及PBC的发病过程中发挥重要作用。Objective To study the relationship between serum interleukin-17（IL-17）,interferon-γ（IFN-γ）,tumor necrosis factor-α（TNF-α） and liver function in patients with autoimmune liver disease（AILD）.Methods 70 patients with AILD were enrolled,including 40 patients with autoimmune hepatitis（AIH）and 30 patients with primary cholestasis cirrhosis（PBC）. The above patients were given intravenous infusion to enhance liver protection,remove jaundice and reduce enzyme. And 20 healthy people in the same period were selected as the control group. The serum levels of IL-17,IFN-γ and TNF-α were detected by ELISA before and after treatment. The serum levels of immunoglobulin G（IgG）and liver function related indexes such as serum total bilirubin（TBIL）,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,glutamyltransferase（γ-GT）were also detected. Results 1）The levels of serum IL-17,TBIL,AST,ALT,γ-GT and IgG in AIH group and PBC group were higher than those in control group before treatment（P〈0.05）. The serum levels of IL-17,TBIL,AST,ALT and IgG in AIH group and PBC group were lower than those before treatment（P〈0.05）.There was no significant difference between the two groups before and after treatment（P〈0.05）. The serum levels of TBIL,AST,ALT and γ-GT in AIH group and PBC group were higher than those in control group after treatment（P〈0.05）. There was no significant difference in the levels of IL-17 and IgG between AIH group and PBC group（P〉0.05）. 2）The levels of serum IFN-γ and TNF-α in the AIH group and the PBC group were higher than those in the control group（P〈0.05）. There was no significant difference between the two groups（P〉0.05）. The levels of serum IFN-γ and TNF-α in the PBC group were lower than those before treatment（P〈0.05）. 3）Serum IL-17 was positively correlated with TBIL,AST,ALT,IgG,γ-GT,IFN-γ and TNF-α in 70 patients with AILD（P〈0.05）. Conclusion The IL-17 expression is significantly higher i

关 键 词：自身免疫性肝病 自身免疫性肝炎 原发性胆汁淤积性肝硬化 白细胞介素17 肝功能 免疫球蛋白 

分 类 号：R575[医药卫生—消化系统]