藏红花酸对TGF-β1刺激的人肝星状细胞LX-2信号转导通路的影响  

Effects of Crocetin on Signal Transduction Pathway of Human Hepatic Stellate Cells Stimulated by TGF-β1

作　　者：杨培青[1] 汪云[1] 梅夏齐 王风秀[1] YANG Pei-qing; WANG Yun; MEI Xia-qi; WANG Feng-xiu(Department of Infectious disease of The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongfiang, 150001, China)

机构地区：[1]哈尔滨医科大学附属第四医院感染科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2018年第17期3230-3234,共5页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金项目(D201076)

摘　　要：目的:研究藏红花酸(crocetin)对转化生长因子-β1(TGF-β1)刺激的人肝星状细胞LX-2信号转导通路的影响。方法:体外培养LX-2细胞,随机设立空白对照组,模型组,藏红花酸低剂量组,藏红花酸中剂量组,藏红花酸高剂量组,用含10%血清的1640培养液培养48 h,MTT法测各组细胞增殖,蛋白免疫印迹测定各组细胞α-SMA蛋白的表达,实时荧光定量PCR法测各组细胞Smad2、Smad3、Smad7mRNA的表达。结果:与对照组比较,TGF-β1刺激后人肝星状细胞LX-2增殖作用明显,且上调α-SMA蛋白、Smad2mRNA、Smad3mRNA表达,下调Smad7mRNA表达(P<0.01)与模型组比较,藏红花酸组均能抑制LX-2细胞增殖,并呈剂量依赖性,高、中剂量组作用明显,能够明显下调α-SMA蛋白、Smad2mRNA、Smad3mRNA表达,上调Smad7mRNA表达,差异具有统计学意义(P<0.05)。结论:藏红花酸对TGF-β1刺激的LX-2细胞中Smad7mRNA具有上调作用,对Smad2、Smad3mRNA具有下调作用,其抗肝纤维化作用可能与抑制TGF-β1/Smads信号转导通路有关。Objective： To study the effects of crocetin on TGF-β1 stimulated signal transduction pathway in human hepatic stellate cells（HSC-LX2）. Methods： HSC-LX2 cells were cultured in vitro and randomly divided into 5 groups： blank group, model group,low-dose crocetin group, medium-dose group, high-dose crocetin group. Then cells proliferation were measured by MTT, the expression of α-SMA was measured by Western blot, the gene expression of Smad2/Smad3/Smad7 m RNA were measured by Real-time PCR. Results：Compared with control group, the model group promotes cells proliferation. It can upregulate the expression of α-SMA protein,Smad2 mRNA and Smad3 mRNA and downregulate the expression of Smad7 mRNA（P〈0.01）. Compared with model group, crocetin group can inhibit the effect of TGF-β1 in a dose-dependent manner（P〈0.05）. The high-dose and medium-dose crocetin groups can downregulate the expression of α-SMA protein, Smad2 mRNA and Smad3 mRNA and upregulate the expression of Smad7 mRNA（P〈0.05）.Conclusions： Crocetin can upregulate the expression of Smad7 mRNA and downregulate the expression of Smad2 mRNA and Smad3 mRNA in LX-2 cells induced by TGF-β1. Thus the anti-hepatic fibrosis mechanism of crocetin may be related to the inhibition of TGF-β1/Smads signal transduction pathway.

关 键 词：肝星状细胞 转化生长因子 藏红花酸 TGF-β1/Smads 

分 类 号：R-33[医药卫生]