胃癌患者外周血调节性T细胞水平与免疫抑制状态及病理特征的关系研究  

Research on Relationship Between Regulatory T Cell Levels in Peripheral Blood and Immunosuppressive Status and Pathological Features in the Patients with Gastric Cancer

作　　者：王海玮[1] 柴丽丽[1] 杨国青[1] 司望利[2] 陈燕[1] WANG Hai-wei;CHAI Li-li;YANG Guo-qin;SI Wang-li;CHEN Yan(Department of Pathology, Xi＇an Central Hospital, Xi＇an, Shaanxi, 710003, China; Department of Gastroenterology, Xi＇an Central Hospital, Xi＇an, Shaanxi, 710003, China)

机构地区：[1]西安市中心医院病理科,陕西西安710003 [2]西安市中心医院消化科,陕西西安710003

出　　处：《现代生物医学进展》2018年第17期3365-3369,共5页Progress in Modern Biomedicine

摘　　要：目的:探讨胃癌患者外周血中CD4^+CD25^+调节性T细胞(Treg)水平与免疫抑制状态和病理特征的关系。方法:选择2016年1月至2017年6月我院收治的胃癌患者73例作为胃癌组,另选同期在本院进行体格检查的健康者41例作为对照组,采用流式细胞仪检测其外周血中CD4^+CD25^+Treg水平,采用酶联免疫吸附试验(ELISA)检测血清γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)水平,分析CD4^+CD25^+Treg水平与IFN-γ、IL-2、IL-4和IL-10的相关性及与病理特征的关系。结果:胃癌组CD4^+CD25^+Treg比例、IL-4和IL-10水平为(19.43±4.36)%、(9.76±2.41)pg/mL和(22.18±5.26)pg/mL,高于对照组的(10.34±2.16)%、(7.16±2.07)pg/mL和(9.52±3.47)pg/mL,差异有统计学意义(P<0.05);胃癌组IFN-γ和IL-2水平为(6.87±2.24)pg/mL和(2.43±0.54)pg/mL,低于对照组的(13.86±3.18)pg/mL和(12.79±2.16)pg/mL,差异有统计学意义(P<0.05)。CD4^+CD25^+Treg比例与IFN-γ和IL-2呈负相关关系(P<0.05),与IL-4和IL-10呈正相关关系(P<0.05)。CD4^+CD25^+Treg比例与胃癌患者的TNM分期、淋巴结转移有关(P<0.05),与病理类型、肿瘤直径、分化程度和肿瘤位置无关(P>0.05)。结论:胃癌患者外周血Treg水平增高,与免疫状态有明显的相关性,其参与了肿瘤的发生与发展过程。Objective： To investigate the relationship between the levels of CD4＋CD25＋regulatory T cells（Treg） in peripheral blood of patients with gastric cancer and the immunosuppressive state and pathological features. Methods： 73 patients with gastric cancer who were treated in our hospital from January 2016 to June 2017 were selected as gastric cancer group, 41 healthy persons who underwent physical examination in our hospital were selected as control group in the same period.The levels of CD4＋CD25＋Treg in peripheral blood were detected by flow cytometry,serum levels of interferon-γ（IFN-γ）, interleukin-2（IL-2）, interleukin-4（IL-4） and interleukin-10（IL-10） were measured by enzyme linked immunosorbent assay（ELISA）, the correlation between CD4＋CD25＋Treg level and IFN-γ, IL-2,IL-4 and IL-10 and the relationship with the pathological features were analyzed. Results： The ratio of CD4＋CD25＋Treg, the levels of IL-4 and IL-10 in the gastric cancer group were（19.43±4.36）%,（9.76±2.41）pg/mL and（22.18±5.26）pg/mL, which were higher than（10.34±2.16）%,（7.16±2.07）pg/mL and（9.52±3.47）pg/mL of the control group, the differences were statistically significant（P〈0.05）.The level of IFN-γ and IL-2 in gastric cancer group were（6.87±2.24）pg/mL and（2.43±0.54）pg/mL, which were lower than（13.86±3.18）pg/m L and（12.79 ±2.16）pg/ml of the control group,the differences were statistically significant（P〈0.05）. The proportion of CD4＋CD25＋Treg was negatively correlated with IFN-γ and IL-2（P〈0.05）, and there was a positive correlation with IL-4 and IL-10（P〈0.05）. The ratio of CD4＋CD25＋Treg was related to TNM stage and lymph node metastasis in gastric cancer patients（P〈0.05）, which was independent of pathological type, tumor diameter, differentiation degree and tumor location（P〉0.05）. Conclusion： The level of Treg in peripheral blood of patients with gastric cancer is higher, and it is closely related to the immu

关 键 词：胃癌 调节性T细胞 免疫抑制状态 相关性 

分 类 号：R735.2[医药卫生—肿瘤]