调强放疗联合局部热疗对晚期肝癌病灶中Mortalin表达的影响及其与细胞增殖、EMT的关系  

Effect of intensity-modulated radiotherapy combined with local hyperthermia on mortalin expression and its relationship with cell proliferation and EMT in advanced liver cancer lesions

作　　者：李东 LI Dong(Department of General Surgery,Xingyuan Hospital,Yulin 719000,China)

机构地区：[1]陕西省榆林市星元医院普外科,陕西榆林719000

出　　处：《海南医学院学报》2018年第20期1842-1845,共4页Journal of Hainan Medical University

基　　金：2016年陕西省自然基金项目(2016JM8080)~~

摘　　要：目的:探讨调强放疗联合局部热疗对晚期肝癌病灶中Mortalin表达的影响及其与细胞增殖、EMT的关系。方法:选取在本院接受治疗的晚期肝癌患者82例作为研究对象,回顾治疗方案并将入组患者分为接受调强放疗的对照组43例、接受调强放疗联合局部热疗的联合治疗组39例。治疗后经腹腔穿刺获取肝癌组织标本,对比组织中Mortalin基因及细胞增殖基因、EMT基因表达量的差异。结果:治疗后,联合治疗组肝癌组织中Mortalin mRNA表达量低于对照组;肝癌组织中Fbxw7mRNA的表达量高于对照组,NCX1、PRMT5、XBP1SmRNA的表达量低于对照组;肝癌组织中Twist、ZEB-1、ZEB-2、Snail、HOXB7mRNA的表达量均低于对照组。Pearson检验发现,肝癌组织中Mortalin基因表达量与细胞增殖基因、EMT基因直接相关。结论:调强放疗联合局部热疗可有效降低Mortalin基因表达量,进一步通过抑制肝细胞增殖及EMT活力等途径优化患者病情。Objective： To investigate the effect of intensity-modulated radiotherapy combined with local hyperthermia on mortalin expression and its relationship with cell proliferation and EMT in advanced liver cancer lesions. Methods A total of 82 patients with advanced liver cancer who were treated in Xingyuan Hospital between February 2016 and September 2017 were chosen as the research subjects, and were divided into the control group （ n =43） who received intensity-modulated radiotherapy, and the combined treatment group （ n =39） who received intensity-modulated radiotherapy combined with local hyperthermia. Abdominal puncture was performed after treatment to obtain liver cancer tissue samples, and the differences in mortalin gene, cell proliferation gene and EMT gene expression in tissues were compared. Results After treatment, mortalin mRNA expression in liver cancer tissues of combined treatment group was lower than that of control group; Fbxw7 mRNA expression in liver cancer tissues was higher than that of control group whereas NCX1, PRMT5 and XBP1S mRNA expression were lower than those of control group; Twist, ZEB-1, ZEB-2, Snail and HOXB7 mRNA expression in liver cancer tissues were lower than those of control group. Pearson test showed that the mortalin gene expression in liver cancer tissues was directly correlated with cell proliferation genes and EMT genes. Conclusions Intensity-modulated radiotherapy combined with local hyperthermia can effectively reduce mortalin gene expression, and further improve patients＇ condition by inhibiting hepatic cell proliferation, EMT activity and other pathways.

关 键 词：晚期肝癌 调强放疗 局部热疗 Mortalin 细胞增殖 EMT 

分 类 号：R735.7[医药卫生—肿瘤]