2001～2014年吉林省麻疹病毒核蛋白基因变异分析  

Gene variation of measles virus nucleoprotein in Jilin Province,China during 2001～2014

作　　者：黄飚[1] 单元春[1] 周剑惠[1] 王爽[2] 田鑫[1] 杨尧[1] 魏雷雷[2] HUANG Biao;SHAN Yuan-chun;ZHOU Jian-hui;WANG Shuang;TIAN Xin;YANG Yao;WEI Lei-lei(Jihn Provtnctal Center for Disease Control and Prevention,Changchun 130062,Jilin Province,China)

机构地区：[1]吉林省疾病预防控制中心吉林省卫计委病毒重点实验室,吉林长春130062 [2]吉林省卫生监测检验中心,吉林长春130062

出　　处：《中国生物制品学杂志》2018年第10期1090-1094,共5页Chinese Journal of Biologicals

基　　金：吉林省卫生计生科研计划课题"吉林省十四年麻疹分子流行病学及病毒变异变迁研究"(2014Z031)

摘　　要：目的分析2001～2014年吉林省麻疹病毒核蛋白(nucleoprotein,N)基因变异情况。方法采用病毒分离、核酸提取及扩增、测序等方法获得麻疹病毒N蛋白基因序列,并通过Bio Edit、Mega等生物信息学软件对所得序列进行变异分析。结果吉林省2005、2006、2009和2014年为麻疹高发年份;麻疹病毒流行株核苷酸变异率为0.59%～1.85%,氨基酸变异率为1.00%～2.67%,氨基酸的第100位点均出现L→I的突变,可能为吉林省特异性位点,2009年第82位点出现S→G的回复性突变,且一直延续至2014年,与第47和122位点的变异处于同一病毒株上,2013和2014年第63(R→I)、69(S→T)、77(R→L)和82位(R→I)点出现一致的突变;2009、2010年麻疹病毒株错义突变率最高;第47、82、122位点的氨基酸变异持续稳定流行多年,且其熵值最高。结论 2001～2014年吉林省麻疹病毒N蛋白基因的核苷酸和氨基酸变异率随年份的推移呈波动上升趋势,建议持续监测关键位点的氨基酸变异情况,结合流行病学资料对麻疹疫情的发展提供预测预警。Objective To analyze the gene variation of measles virus nucleoprotein （N） in Jitin Province, China from 2001 to 2014. Methods The N gene sequence of measles virus was obtained by virus isolation, extraction and amplification of RNA and sequencing, of which the variation was analyzed by using BioEdit and Mega software. Results The incidences of measles were high in 2005, 2006, 2009 and 2014. The variation rates of nucleotide （nt） and amino acid （aa） of epidemic strains of measles virus were 0. 59% -1. 85% and 1.00% N 2. 67% respectively. The mutation of LI appeared at aal00 which might be the specific site in Jilin Province in various years. However, the back mutation of S → G appeared at aa82 in 2009 and last to 2014, which was localized in the same strain with those at aa 47 and aa122. The mutations at aa63 （R→I）, aa69 （S→T）, aa77 （R→L） and aa82 （R→I） appeared in 2013 and 2014. The missense mutation rates were the highest in 2009 and 2010. The mutations at aa47, aa82 and aa122 were epidemic for many years, of which the entropies were higher than those at other sites. Conclusion Both the variation rates of nt and aa of measles virus strains in Jilin Province during 2001 - 2014 increased year by year. The amino acid variation in the key sites of should be monitored continuously to provide an early warning for epidemic of measles in combination with epidemiological data.

关 键 词：麻疹病毒 核蛋白 基因变异 

分 类 号：R373.11[医药卫生—病原生物学]