Muscarinic acetylcholine receptor M1 mediates prostate cancer cell migration and invasion through hedgehog signaling  

作　　者：Qing-Qing Yin Lin-Hui Xu Mi Zhang Chen Xu Qing-Qing Yin;Lin-Hui Xu;Mi Zhang;Chen Xu(Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China)

机构地区：Institute of Life Sciences,Chongqing Medical University,Chongqing 400016,China.

出　　处：《Asian Journal of Andrology》2018年第6期608-614,共7页亚洲男性学杂志（英文版）

基　　金：Tnis workwas supportedby the Natural Science Foundation of Chongqing （CSTC, 2009BA5081）.

摘　　要：The autonomic nervous system contributes to prostate cancer proliferation and metastasis. However, the exact molecular mechanism remains unclear. In this study, muscarinic acetylcholine receptor M1 （CHRM1） expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides. PC-3, LNCaP, and A549 cells were treated with pirenzepine or carbachol, and the cell migration and invasion abilities were evaluated. Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 （GLI1）, patched 1 （PTCH1）, and sonic hedgehog （SHH） expression levels. High expression of CHRM1 was found in early-stage human prostate cancer tissues. In addition, the selective CHRM1 antagonist pirenzepine inhibited PC-3, LNCaP, and A549 cell migration and invasion, but the agonist carbachol promoted the migration and invasion of these three cell lines. Muscarinic signaling can be relayed by hedgehog signaling. These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hed^eho~ si^nalin~ ~athwav.The autonomic nervous system contributes to prostate cancer proliferation and metastasis. However, the exact molecular mechanism remains unclear. In this study, muscarinic acetylcholine receptor M1 （CHRM1） expression was measured via immunohistochemical analysis in human prostate cancer tissue array slides. PC-3, LNCaP, and A549 cells were treated with pirenzepine or carbachol, and the cell migration and invasion abilities were evaluated. Western blotting and quantitative real-time PCR were performed to measure GLI family zinc finger 1 （GLI1）, patched 1 （PTCH1）, and sonic hedgehog （SHH） expression levels. High expression of CHRM1 was found in early-stage human prostate cancer tissues. In addition, the selective CHRM1 antagonist pirenzepine inhibited PC-3, LNCaP, and A549 cell migration and invasion, but the agonist carbachol promoted the migration and invasion of these three cell lines. Muscarinic signaling can be relayed by hedgehog signaling. These data show that CHRM1 is involved in the regulation of prostate cancer migration and invasion through the hed^eho~ si^nalin~ ~athwav.

关 键 词：hedgehog signaling muscarinic acetylcholine receptor M1 （CHRM1） METASTASIS prostate cancer 

分 类 号：Q959.837[生物学—动物学] TP273[自动化与计算机技术—检测技术与自动化装置]