高脂饮食对雄性小鼠睾丸生精功能的影响  

The effect of high fat diet on spermatogenesis in male mice

作　　者：杨雪梅[1] 李俊[1] 刘芳[1] 刘元科 武艳[2] YANG Xue-mei;LI Jun;LIU Fang;LIU Yuan-ke;WU Yan(Reproductive Medicine Department of the First Hospital of Hebei Medical University,Shijiazhuang 050031,Hebei,China)

机构地区：[1]河北医科大学第一医院生殖医学科,河北石家庄050031 [2]河北医科大学第一医院检验科,河北石家庄050031

出　　处：《广东医学》2018年第19期2869-2873,共5页Guangdong Medical Journal

基　　金：河北省医学科学研究重点课题(编号:20150654)

摘　　要：目的探讨高脂饮食对雄性小鼠生精功能的影响。方法将16只C57BL/6J雄性小鼠随机分为正常饮食组和高脂饮食组,连续喂养16周后,检测血清三酰甘油、总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、睾酮和雌二醇水平,并对睾丸组织进行病理学检查和电镜观察。结果与正常饮食组相比,高脂饮食组小鼠的血清三酰甘油、总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、雌二醇明显升高,睾酮明显降低,精子浓度与活力均明显降低。HE染色示正常饮食组小鼠睾丸组织排列紧密,曲细精管排列整齐,结构完整,由管壁至管腔各级生精细胞层次分明;高脂饮食组小鼠曲细精管排列松散,生精细胞数目减少,结构肿胀。电镜示高脂饮食组睾丸组织中脂滴增多,部分生精细胞颜色变深,胞质固缩,粗面内质网扩张,膜旁核糖体脱落。结论高脂饮食可影响小鼠生精微环境,导致精液质量下降。Objective To investigate the effect of high fat diet on spermatogenesis in male mice. Methods Sixteen male C57 BL/6 J mice were randomly divided into normal diet group and high fat group. After 16 weeks feeding,blood samples were taken from the heart to detect serum triglyceride,total cholesterol,low density lipoprotein cholesterol,high density lipoprotein cholesterol,testosterone and estradiol; as the testicular tissues were collected for pathological examination and electron microscopy. Results Serum triglyceride,total cholesterol,low density lipoprotein cholesterol,high-density lipoprotein cholesterol and estradiol were significantly higher in high-fat diet group than those in normal diet group,while testosterone significantly decreased. HE staining showed that the testis tissues in normal diet group were tightly arranged,the seminiferous tubules were orderly arranged with complete structure,and the arrangment of spermatogenic cells at all stages were distinct from the tube wall to the lumen. In the high-fat diet group,the seminiferous tubules were loosely arranged,the number of spermatogenic cells decreased,and the structure swelled. Electron microscopy showed that the fat droplets in testicular tissue increased,some spermatogenic cells became darker,the cytoplasm was contracted,the rough endoplasmic reticulum expanded,and the ribosomal bodies were detached. Conclusion High fat diet can affect the spermatogenesis microenvironment of mice and lead to the decrease of semen quality.

关 键 词：高脂饮食 C57BL/6J雄性小鼠 生精功能 

分 类 号：R589.2[医药卫生—内分泌] R698[医药卫生—内科学]