番木瓜果实转录组数据组装及基因功能注释  

Transcriptome Data Assembly and Gene Function Annotation of Carica papaya Fruits

作　　者：蒋边 潘嘉琪 叶柳清 陆丽施 袁长春 刘锴栋 Jiang Bian 1, Pan Jiaqi 1, Ye Liuqing 1, Lu Lishi 1, Yuan Changchun 1, Liu Kaidong 1(1 Life Science and Technology School, Lingnan Normal University, Zhanjiang, 524048; 2 College of Bioscience and Technology, Hunan Agriculturl University, Changsha, 41012)

机构地区：[1]岭南师范学院生命科学与技术学院,湛江524048 [2]湖南农业大学生物科学技术学院,长沙410128

出　　处：《分子植物育种》2018年第21期6945-6954,共10页Molecular Plant Breeding

基　　金：国家自然科学基金(31201586);广东省科技计划(2016A040402046; 2017A040403069);广东省"扬帆计划"重点人才项目;湛江市科技计划(2016A03016);岭南师范学院自然科学研究重点项目(LZL1507)共同资助

摘　　要：为了获得番木瓜果实转录组数据及预测关键基因功能。本研究通过Illumina HiSeq TM 2500高通量测序技术平台对番木瓜果实中提取的总RNA进行测序,获得了平均值为36 770 860.67个原始reads。经过滤、从头组装和拼接获得了54 941条高质量Unigene,平均长度为921 bp,N50的值为1 770。对所得Unigene进行不同数据库注释,30 592个和24 467个Unigene分别在NR和Swiss-Prot数据库有同源比对信息,确定了番木瓜同源序列所属物种;在KOG数据库中共有29 974个Unigene被注释上25种功能,其中涵盖基因最多的是一般功能预测;114 207个Unigene被注释到生物学过程、细胞组分及分子功能的GO数据库三大类别的48个功能组,其中大量Unigene与细胞过程、代谢过程、细胞部分、细胞催化及结合相关;根据KEGG数据库,可将11 766个Unigene定位到130个代谢途径分支,其中大部分的基因都与氨基酸、碳水化合物、脂类以及核苷酸的代谢相关。本研究结果将为进一步开展番木瓜果实的相关代谢途径、激素信号传导途径以及生物活性物质的合成途径中关键基因的挖掘提供理论参考。The study aimed to obtain the transcriptome data of papaya and predict the functions of key genes. The total RNA extracted from the papaya fruit was sequenced by using high-throughput sequencing platform Illumina HiSeqTM 2500, and an average of 36 770 860.67 raw reads were generated. 54 941 high quality unigenes were obtained after filtration, De novo assembly and splicing of which the mean size was 921 bp and the value of N50 was 1 770. All unigenes were annotated by different databases. 30 592 and 24 467 unigenes were detected with homologous comparison information in NR and Swiss-Prot databases respectively, which therefore identified the species of papaya homologous sequences. A total of 29 974 tmigenes were annotated with 25 functions in KOG database, and the most common function with gene was general function prediction. 114 207 unigenes were an not- ated into 48 function groups in 3 categories of biological process, cellular component and molecular function in GO database. Among them, a large number of unigenes were associated with metabolic processes, cellular processes,cell part, cells catalytic activity and binding. According to the KEGG database, 11 766 unigenes could be localized to 130 metabolic pathways branches, and most of which were related to amino acid, carbohydrate, lipid and nucleotide metabolism. The results of this study might provide a basis for further mining of key genes of papaya fruits in the associated metabolic pathway, hormonal signaling pathways and the synthesis of biologically active substances.

关 键 词：番木瓜 转录本 转录组测序 功能注释 

分 类 号：S667.9[农业科学—果树学]