通腑泄热法对大鼠肝脏缺血再灌注损伤的保护作用  

Protective effect of tongfu xiere method on hepatic ischemia reperfusion injury in rats

作　　者：李伟涛 翟淑萍 章小兵 苑伟 张北平 王百林 Li Weitao;Zhai Shuping;Zhang Xiaobing;Yuan Wei;Zhang Beiping;Wang Bailin(Department of General Surgery,Guangzhou Red Cross Hospital,Medical College of Jinan University,Cruangzhou 510220,China)

机构地区：[1]暨南大学医学院附属广州红十字会医院普通外科,广州510220 [2]贵州医科大学

出　　处：《腹部外科》2018年第5期365-368,共4页Journal of Abdominal Surgery

基　　金：广东省自然科学基金(2015A03313364)

摘　　要：目的探讨通腑泄热法中药对大鼠肝脏缺血再灌注损伤的保护作用及其机制。方法采用肝门部血流阻断法制备大鼠肝缺血再灌注模型。SD大鼠150只,按随机分组原则分为缺血再灌注通腑泻热方中药组(TCM组,n=75)和缺血再灌注对照组(Control组,n=75),各组按缺血再灌注损伤后1、3、6h分成3个亚组,各亚组25只大鼠,在各时间点抽取两组大鼠下腔静脉血液,检测血清中白细胞介素6(interleukin 6,IL-6)水平,取肝组织检测肝组织内的过氧化氢酶(catalase,CAT)、髓过氧化物酶(myeloperoxidase,MPO)含量,评估两组不同时间点肝组织缺血再灌注损伤指标变化。结果 TCM组和Control组在肝缺血再灌注后1、3、6h的IL-6(ng/L)的表达分别为:39.68±1.10、22.73±1.85、35.83±3.41和47.7±0.35、48.22±0.66、41.29±1.29;CAT(U/mgprot):78.17±1.26、168.89±2.18、245.33±3.85和59.20±2.24、68.79±3.08、82.86±4.33;MPO(U/克组织湿重):8.66±1.07、16.15±1.45、25.96±3.01和16.84±1.28、36.51±2.03、55.99±4.70。与Control组相比,TCM组在3个时间点IL-6、MPO含量降低,CAT升高,差异均有统计学意义(均P<0.05)。结论通腑泄热法中药通过抑制急性炎症介质细胞因子如IL-6及MPO的释放、以及保护CAT活性,加速氧自由基清除,以此对大鼠肝缺血再灌注损伤起保护作用。Objective To investigate the protective effect and mechanism of TongFu XieRe Method on hepatic ischemia-reperfusion injury in rats.Methods The liver ischemia-reperfusion model was established by blocking the hepatic portal vein.150 SD rats were randomly divided into two groups：ischemia-reperfusion reperfusion and diarrhea fever Chinese medicine group（TCM group,n=75）and ischemia-reperfusion control group（control group,n=75）.Both groups were divided into three subgroups at 1 h,3 h,and 6 hafter ischemia-reperfusion injury（n=25 each）.At each time point,the inferior vena cava blood was drawn from both groups to measure serum interleukin-6（IL-6）levels,and the liver tissues were obtained to detect the contents of catalase（CAT）and myeloperoxidase（MPO）.The changes in parameters for liver ischemia and reperfusion injury at different time points were evaluated.Results At different time points（1,3 and 6 h）in TCM and control groups,the expression levels of IL-6（ng/L）were 39.68±1.10,22.73±1.85 and 35.83±3.41,and 47.70±0.35,48.22±0.66 and 41.29±1.29;CAT contents（U/mgprot）were 78.17±1.26,168.89±2.18 and 245.33±3.85 and 59.20±2.24,68.79±3.08 and 82.86±4.33;MPO（U/wet weight）contents were 8.66±1.07,16.15±1.45 and 25.96±3.01,and 16.84±1.28,36.51±2.03 and 55.99±4.70,respectively.As compared with the control group,the levels of IL-6,MPO decreased CAT raised in the TCM group at three time points（P〈0.05）.Conclusions The traditional Chinese medicine for removing heat of Tongqiao can protect against hepatic ischemia-reperfusion injury in rats probably by inhibiting the release of cytokines such as IL-6 and MPO to protect CAT activity and accelerating the clearance of oxygen free radicals.

关 键 词：通腑泻热法 肝缺血再灌注 过氧化氢酶 白细胞介素6 髓过氧化物酶 

分 类 号：R575[医药卫生—消化系统]